{"title":"Immobilization of Papain on Siliceous Mesocellular Foam","authors":"Nie Zuoren","doi":"10.3724/sp.j.1077.2009.00702","DOIUrl":null,"url":null,"abstract":"Siliceous mesocellular foam(MCF)is employed as carriers in the immobilization of papain,and the properties and the stabilities of the immobilized enzyme are investigated in detail.The results show that the amount of papain immobilized on MCF reaches 334mg/g MCF and the activity of immobilized papain is 95.3% of that of the free papain.The Km of immobilized and free papain are 6.99×10-3mol/L and 6.71×10-3mol/L,respectively.The thermal and storage stabilities of immobilized enzyme are improved due to the microenvironment and the shield of the mesopores.MCF can be considered as excellent supports for papain immobilization.","PeriodicalId":37461,"journal":{"name":"Cailiao Daobao/Materials Review","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cailiao Daobao/Materials Review","FirstCategoryId":"1089","ListUrlMain":"https://doi.org/10.3724/sp.j.1077.2009.00702","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Materials Science","Score":null,"Total":0}
引用次数: 1
Abstract
Siliceous mesocellular foam(MCF)is employed as carriers in the immobilization of papain,and the properties and the stabilities of the immobilized enzyme are investigated in detail.The results show that the amount of papain immobilized on MCF reaches 334mg/g MCF and the activity of immobilized papain is 95.3% of that of the free papain.The Km of immobilized and free papain are 6.99×10-3mol/L and 6.71×10-3mol/L,respectively.The thermal and storage stabilities of immobilized enzyme are improved due to the microenvironment and the shield of the mesopores.MCF can be considered as excellent supports for papain immobilization.
期刊介绍：
Materials Reports (MR) publishes articles which focus on, but are not limited to, the following areas. I. Innovative functional materials, including new energy materials, optical/electro-nic/magnetic materials, biomedical materials, materials for environment protection and remediation, etc. II. Advanced structural materials, including structural ceramics, metal materials, civil engineering materials, surface engineering and material connection, composite materials, polymer materials, etc. It is hereby declared that since January 2019, the English title of the Chinese journal Materials Review (Cailiao Daobao) has been changed to Materials Reports, and its original English title Materials Review is invalid. We appreciate your support and look forward your sustained attention to our journal.
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