Mingle Cao, Xinglin Jiang, Haibo Zhang, M. Xian, Xin Xu, Wei Liu
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引用次数: 0
Abstract
Nitrilases can hydrolyze nitrile efficiently under mild conditions. For their low pollution and cost, the enzymatic methods with nitrilases are widely used in agriculture, industry, biomedicine and environment protection. Nitrilases with broad substrate specificity are lacked in the nitrilase market. In this study, Berthelot method and high performance liquid chromatography were used to separate and identify a new strain of nitrilase Pantoea sp. 1-2, which can hydrolyze acetonitrile, 4-hydroxyphenylcyanide and 3-hydroxypropionitrile. The strain produced 16.45 U/mL nitrilase with ammonium sulfate as nitrogen source. The enzyme remained 80% activity at pH 6.6-7.6, temperature 22-45 °C, staying stable after 30 h incubation. The strain would be of great value in industrial utilization. Fig 7, Ref 21
应用与环境生物学报Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.20
自引率
0.00%
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4458
期刊介绍：
The “Chinese Journal of Applied and Environmental Biology” is a national scientific and technological journal published both at home and abroad. It was started in 1995 and became bimonthly in 1999, and now it is a core journal for applied and environmental biology in China. It aims to introduce and exchange the new achievements, new technologies, new methods and new progresses made from the research fields of biology and its related disciplines both in China and abroad; enhance scientific and technological research serving for the development and construction of national economy, and improvement of people’s living standards.
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