O658Detection of Metabolites from n -Hexadecane Degradation by Strain Dietzia sp. DQ12-45-1b: O658Detection of Metabolites from n -Hexadecane Degradation by Strain Dietzia sp. DQ12-45-1b

Abstract

This experiment optimized the extraction and detection methods of biosurfactant produced by the strain Dietzia sp. DQ12-45-1b, and studied biosurfactant products of the strain growing on n-hexadecane for different cultural time. Ethyl acetate was the best extraction solvent with the highest metabolite recovery effi cient. The mixture of hexane : ammonia : isopropanol = 2:1:4 (V:V:V) was used as the best solvent system in thin layer chromatography analysis. The strain DQ12-45-1b produced glycolipid biosurfactant, with the biosurfactant concentration of the culture broth positively correlated with the incubation time. The content ratio of crude extracts in emulsifi ed hexadecane layer and aqueous medium was 4.16:1. A glycolipid was detected in the emulsifi ed hexadecane layer but not in aqueous phase. Fig 5, Tab 1, Ref 15