Comparison of different methods for RNA extraction from Capparis spinosa.

Abstract

Capparis spinosa is a shrub with high cultivation and economic value in desert and semi-desert areas,and it is a rare species for studying plant stress resistance.An effective method of isolating total RNA from the leaves of wild Capparis spinosa is developed.RNA is extracted by the methods of Trizol regent,CTAB and SDS by combining with 70% acetone.After separately analyzing the rate of yield,purity,quality,electrophoretograms and RT-PCR production,an improved effective SDS method is preliminary developed to extract the total RNA from Capparis spinosa leaves.The A260/A280 value of RNA extracted with this method varies in a range of 1.88~1.94,and the electrophoretograms are integrated.This RNA extraction method is characterized by the high yield,short duration,low cost,etc.,and can be used in some experiments,such as RT-PCR,cDNA library construction and northern hybridization.