Effect of ethanol on the mRNA expression of neuropeptide receptor and apoptosis-related genes in human bone marrow mesenchymal stem cells

中华实验外科杂志 Pub Date : 2013-04-08 DOI:10.3760/CMA.J.ISSN.1001-9030.2013.04.048

Jing Kai, Li Chencheng, W. Yisheng, Zuohe Song, Li Yue-bai

{"title":"Effect of ethanol on the mRNA expression of neuropeptide receptor and apoptosis-related genes in human bone marrow mesenchymal stem cells","authors":"Jing Kai, Li Chencheng, W. Yisheng, Zuohe Song, Li Yue-bai","doi":"10.3760/CMA.J.ISSN.1001-9030.2013.04.048","DOIUrl":null,"url":null,"abstract":"Objective To observe the effect of ethanol on the mRNA expression of neuropeptide or its receptor and apoptosis-reiated genes in human bone marrow mesenclymal stem cells （hBMSCs）. Meth- ods The hBMSCs were cultivated and isolated by adherence and density gradient centfifugation, and iden- tifided by flow cytometry. The hBMSCs at the third generations were randomly divided into two groups. In the experimental group, the cells were treated with 0. 09 mol/L ethanol. In the control group, the cells were nor- mally cultured without ethanol. The mRNA expression levels of Peroxisom proliferator-activeted receptor-γ （PPAR-γ）, Calcitonin gene-related peptide receptor （CGRPR）, Runt-related transcription factor （Runx2）, B cell lymphoma-2 （bel-2） and Cysteine aspartic acid specific protease-3 （ Caspase-3 ） in hBMSCs were detected by using real-time quantitative reverse transcription-polymerase chain reaction （RT-qPCR） after culture for 11 days. Results The expression levels of CGRPR, Runx2 and bcl-2 mRNA in the experimen- tal group were significantly lower than those in the control group （for CGRPR, 1.90E-01 ± 4. 12E-02 vs. 3.27E-01 ± 8. 24E-02,t = 6. 66 ,P 〈 O. 05; for Runx, 2 1.28E-01 ± 3.18E-02 vs. 2. 50E-01 ± 5.33E-02, t =8.80,P〈0.05; for bcl-2, 1.65E-01 ±3.9gE-02 vs. 2.95E-01 ±6. TgE-O2,t =7.35,P 〈0.05）, but those of PPAR-±/and Caspase-3 mRNA were increased significantly （for Caspase-3, 5.27E-01 ± 1.26E-01 vs. 3.17E-O1 ± 7.46E-02, t = 6. 39, P 〈 0. 05 ; for PPAR-γ, 2. 90E-01 ± 6. 74E-02 vs. 1.15E-01 _± 3.36E-02 ,t = 10. 38,P 〈 0. 05 ）. Conclusion The ethanol can upregulate the exprression of apoptosis-re- lated gene and adipogenic gene in hBMSCs and downregulate the expression of neuropeptide factors and an- ti-apoptosis gene, resulting in the apoptosis and adipogeneic differetiantion of the cells and inhibiting osteo-genic differentiantion. \n \nKey words: \nBone marrow mesenclymal stem cells; Ethanol; B cell lymphoma-2; Caspase-3","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"30 1","pages":"793-795"},"PeriodicalIF":0.0000,"publicationDate":"2013-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华实验外科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2013.04.048","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Objective To observe the effect of ethanol on the mRNA expression of neuropeptide or its receptor and apoptosis-reiated genes in human bone marrow mesenclymal stem cells （hBMSCs）. Meth- ods The hBMSCs were cultivated and isolated by adherence and density gradient centfifugation, and iden- tifided by flow cytometry. The hBMSCs at the third generations were randomly divided into two groups. In the experimental group, the cells were treated with 0. 09 mol/L ethanol. In the control group, the cells were nor- mally cultured without ethanol. The mRNA expression levels of Peroxisom proliferator-activeted receptor-γ （PPAR-γ）, Calcitonin gene-related peptide receptor （CGRPR）, Runt-related transcription factor （Runx2）, B cell lymphoma-2 （bel-2） and Cysteine aspartic acid specific protease-3 （ Caspase-3 ） in hBMSCs were detected by using real-time quantitative reverse transcription-polymerase chain reaction （RT-qPCR） after culture for 11 days. Results The expression levels of CGRPR, Runx2 and bcl-2 mRNA in the experimen- tal group were significantly lower than those in the control group （for CGRPR, 1.90E-01 ± 4. 12E-02 vs. 3.27E-01 ± 8. 24E-02,t = 6. 66 ,P 〈 O. 05; for Runx, 2 1.28E-01 ± 3.18E-02 vs. 2. 50E-01 ± 5.33E-02, t =8.80,P〈0.05; for bcl-2, 1.65E-01 ±3.9gE-02 vs. 2.95E-01 ±6. TgE-O2,t =7.35,P 〈0.05）, but those of PPAR-±/and Caspase-3 mRNA were increased significantly （for Caspase-3, 5.27E-01 ± 1.26E-01 vs. 3.17E-O1 ± 7.46E-02, t = 6. 39, P 〈 0. 05 ; for PPAR-γ, 2. 90E-01 ± 6. 74E-02 vs. 1.15E-01 _± 3.36E-02 ,t = 10. 38,P 〈 0. 05 ）. Conclusion The ethanol can upregulate the exprression of apoptosis-re- lated gene and adipogenic gene in hBMSCs and downregulate the expression of neuropeptide factors and an- ti-apoptosis gene, resulting in the apoptosis and adipogeneic differetiantion of the cells and inhibiting osteo-genic differentiantion. Key words: Bone marrow mesenclymal stem cells; Ethanol; B cell lymphoma-2; Caspase-3

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

乙醇对人骨髓间充质干细胞神经肽受体和凋亡相关基因mRNA表达的影响

目的观察乙醇对人骨髓间充质干细胞(hBMSCs)中神经肽或其受体mRNA表达及凋亡相关基因的影响。方法采用贴壁和密度梯度离心培养分离hBMSCs，流式细胞术鉴定hBMSCs。第三代hBMSCs随机分为两组。实验组细胞经0。09 mol/L乙醇。在对照组中，细胞在不加乙醇的情况下正常培养。培养11天后，采用实时定量逆转录聚合酶链反应(RT-qPCR)检测hBMSCs中过氧化物酶增殖物激活受体-γ (PPAR-γ)、降钙素基因相关肽受体(CGRPR)、runt相关转录因子(Runx2)、B细胞淋巴瘤-2 (bel-2)和半胱氨酸天冬氨酸特异性蛋白酶-3 (Caspase-3) mRNA表达水平。结果实验组CGRPR、Runx2、bcl-2 mRNA表达水平显著低于对照组(CGRPR为1.90E-01±4)。12E-02 vs. 3.27E-01±824E-02,t = 6。66, p < 0.05;对于Runx, 1.28E-01±3.18E-02 vs. 2。50E-01±5.33E-02, t =8.80,P < 0.05;bcl-2为1.65E-01±3.9gE-02 vs. 2.95E-01±6。TgE-O2,t =7.35,P < 0.05)，而PPAR-±/和Caspase-3 mRNA的表达量显著升高(Caspase-3, 5.27E-01±1.26E-01 vs. 3.17 e- 01±7.46E-02, t = 6)。39, p < 0;05;对于PPAR-γ， 2。90e-01±6。74E-02 vs. 1.15E-01 _±3.36E-02,t = 10。38, p < 0。05 ）. 结论乙醇可上调hBMSCs中凋亡相关基因和成脂基因的表达，下调神经肽因子和抗凋亡基因的表达，导致细胞凋亡和成脂分化，抑制成骨分化。关键词:骨髓间充质干细胞;乙醇;B细胞淋巴瘤-2;Caspase-3

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊