Expression of vascular adhesion protein-1 in severe hemorrhagic shock and resuscitation in rats

Abstract

Objective To observe the expression and activity changes of vascular adhesion protein-1(VAP-1)in the small intestine and serum of rats during severe hemorrhagic shock and resuscitation,and to study its influence on shock prognosis.Methods Fifty rats were evenly randomized into sham group,hemorrhagic shock group,shock resuscitation group,control recovery group and the experimental recovery group.Rat models of severe hemorrhagic shock and resuscitation were established.Before shock,1hour after shock and 1hour after resuscitation,the expressions of VAP-1protein and mRNA in the intestinal tissues of rats were examined by Western blotting analysis and real-time RT-PCR,respectively;and the serum levels of VAP-1and its activities were determined by ELISA kit.Rats in the experimental recovery group was resuscitated by injection of 20mg/kg2-bromoethylamine and those in the control recovery group were given 1 mL/kg normal saline,and then the blood pressure,intestinal mucosa injury(Chiu's score),small intestinal epithelial cell apoptosis(TUNEL detection)and 24-hour survival rates were compared between the two recovery groups.Results The intestinal VAP-1protein and mRNA expressions and the serum VAP-1and its activities in the severe hemorrhagic shock group were significantly higher than those in the sham shock group(P0.05).Compared with the shock group,the above parameters were decreased in the recovery group,but were still higher than those in the sham group.Compared with the saline control group,20mg/kg 2-bromoethylamine significantly increased the blood pressure of animals 1hand 24 hafter recovery(P=0.010,0.039),significantly improved the Chiu's score and apoptosis index of small intestinal epithelial cells(P=0.022,P=0.002),and improved the 24-hour survival rates of rats(90%to 60%).Conclusion The levels of VAP-1and its activities are increased in severe hemorrhagic shock rats,and fluid resuscitation can inhibit this increase.Inhibition of VAP-1activities can improve the low blood pressure,intestinal mucosa injury and apoptosis of small intestinal mucosa cells after the severe hemorrhagic shock and resuscitation,improving the 24-hour survival rates of rats.