Screen and preliminary function study of fetal liver specific oncofetal RNA AK003710

Shao-bing Wang, Shuhan Sun, F. Wang
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Abstract

Objective To screen for a fetal liver specific oncofetal RNA and to study its effect on the proliferation and mobility of hepatocellular carcinoma(HCC)cells.Methods By overlapping the microarray results of mouse fetal liver and regenerated liver following partial hepatectomy(PH),we obtained several candidate lncRNAs which were highly expressed in both fetal liver and regenerated liver tissues,and then the expression of these candidate lncRNAs in HCC tissue were also detected.The most differentially expressed lncRNA in the mouse HCC tissues as detected by real-time PCR were chosen for further research.By real-time PCR,the expression of those lncRNA in fetal liver and regenerated liver tissue was verified.For the function study,EdU labeling system and Transwell experiment were carried out to determine the proliferation ability and mobility after knocking down the lncRNA by siRNA transfection in HCC cell line Hepa1-6.Results Seven candidate lncRNAs which were highly expressed in both fetal liver and regenerated liver tissues were obtained;3of them were overexpressed in HCC tissues compared with normal liver tissues,3had no differential expression,and 1 was undetectable.Among the 3overexpressed lncRNAs,lncRNA-AK003710 was the most differentially expressed lncRNA according to microarray and realtime PCR results.And then we detected that lncRNA-AK003710 was overexpressed in the fetal liver and 3kinds of injured liver tissues.Furthermore,the proliferation ability and mobility of Hepa1-6were impaired after knocking down lncRNA-AK003710 in HCC cell line Hepa1-6.Conclusion lncRNA-AK003710 is overexpressed in the fetal liver,regenerated liver tissues,and HCC tissues;therefore it is an oncofetal RNA that can regulate the proliferation and invasion of HCC cells and may be a potential therapeutic target for HCC.
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胎肝特异性癌胎RNA AK003710的筛选及初步功能研究
目的筛选胎儿肝特异性癌胎RNA,研究其对肝细胞癌(HCC)细胞增殖和移动性的影响。方法将部分肝切除(PH)后小鼠胎肝和再生肝的微阵列结果进行重叠,获得在胎肝和再生肝组织中均高表达的几个候选lncRNAs,并检测这些候选lncRNAs在HCC组织中的表达。选择real-time PCR检测到的小鼠HCC组织中差异表达最多的lncRNA进行进一步研究。通过实时荧光定量PCR验证了这些lncRNA在胎肝和再生肝组织中的表达。在功能研究方面,我们通过EdU标记系统和Transwell实验,检测转染siRNA敲低lncRNA后肝癌细胞株Hepa1-6的增殖能力和迁移能力。结果获得7个候选lncrna,在胎肝和再生肝组织中均有高表达,其中3个在HCC组织中与正常肝组织相比过表达,3个无差异表达,1个未检出。在这3个过表达的lncRNA中,根据微阵列和实时PCR结果,lncRNA- ak003710是差异表达最多的lncRNA。然后我们检测到lncRNA-AK003710在胎儿肝脏和3种损伤肝组织中过表达。此外,敲低lncRNA-AK003710后,肝癌细胞系Hepa1-6的增殖能力和移动性受到损害。结论lncRNA-AK003710在胎肝、再生肝组织和HCC组织中均有过表达,是一种能调控HCC细胞增殖和侵袭的癌胎RNA,可能是HCC潜在的治疗靶点。
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来源期刊
海军军医大学学报
海军军医大学学报 Medicine-Medicine (all)
CiteScore
0.50
自引率
0.00%
发文量
14752
期刊介绍: Founded in 1980, Academic Journal of Second Military Medical University(AJSMMU) is sponsored by Second Military Medical University, a well-known medical university in China. AJSMMU is a peer-reviewed biomedical journal,published in Chinese with English abstracts.The journal aims to showcase outstanding research articles from all areas of biology and medicine,including basic medicine(such as biochemistry, microbiology, molecular biology, genetics, etc.),clinical medicine,public health and epidemiology, military medicine,pharmacology and Traditional Chinese Medicine),to publish significant case report, and to provide both perspectives on personal experiences in medicine and reviews of the current state of biology and medicine.
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