Estrogen replacement increases β-arrestin2 expression in cardiac tissues in ovariectomized rats

Q4 Medicine 海军军医大学学报 Pub Date : 2015-01-01 DOI:10.3724/SP.J.1008.2015.00634

Fan Hao, X. Tan, Wei-Zhong Wang, Mingjuan Xu

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Abstract

Objective To observe the expression ofβ-arrestin2 in cardiac tissues of ovariectomzied(OVX)rats,and to explore the role ofβ-arrestin2 in estrogen-mediated cardiovascular protection in OVX rats.Methods OVX rats were evaluated by monitoring the serum levels of estrogen and the ratio of uterine weight to body weight.The cardiovascular function of rats was assessed by measuring the ratio of heart weight to body weight,blood pressure and heart rate after estrogen replacement treatment for 4weeks,Western blotting analysis was performed to detect the protein expression ofβ-arrestin1,β-arrestin2 and angiotensin receptor type 1(AT1R)in the cardiac tissues.Results Compared with rats in the sham group,the serum estrogen level and the ratio of uterine weight to body weight in OVX rats were decreased significantly(P0.05);meanwhile,the cardiovascular parameters including blood pressure,heart rate and the ratio of heart weight to body weight were significantly increased(P0.05)in OVX rats,and they were attenuated after treatment with estrogen replacement.Compared with the sham controls,the cardiac expression ofβ-arrestin2 was decreased significantly in OVX rats(P0.05),with up-regulated estrogen expression and unchanged expression ofβ-arrestin1 and AT1R.Conclusion β-Arrestin2 expression is decreased in the cardiac tissues of OVX rats,which can be increased by estrogen replacement therapy.

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雌激素替代增加去卵巢大鼠心脏组织中β-arrestin2的表达

目的观察β-arrestin2在卵巢切除(OVX)大鼠心脏组织中的表达，探讨β-arrestin2在雌激素介导的OVX大鼠心血管保护中的作用。方法通过监测OVX大鼠血清雌激素水平和子宫重量与体重之比对其进行评价。雌激素替代治疗4周后，通过测量大鼠的心重与体重之比、血压和心率来评估大鼠的心血管功能，采用Western blotting分析检测心脏组织中β-arrestin1、β-arrestin2和血管紧张素受体1型(angiotensin receptor type 1, AT1R)蛋白的表达。结果与假手术组比较，OVX大鼠血清雌激素水平、子宫重量/体重比均显著降低(P0.05); OVX大鼠血压、心率、心重/体重比均显著升高(P0.05)，经雌激素替代治疗后均有所降低。与假对照组相比，OVX大鼠心脏β-arrestin2表达显著降低(P0.05)，雌激素表达上调，β-arrestin1和AT1R表达不变。结论OVX大鼠心脏组织中β-Arrestin2表达降低，雌激素替代治疗可使其表达升高。

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来源期刊

海军军医大学学报 Medicine-Medicine (all)

CiteScore

0.50

自引率

0.00%

发文量

14752

期刊介绍： Founded in 1980, Academic Journal of Second Military Medical University(AJSMMU) is sponsored by Second Military Medical University, a well-known medical university in China. AJSMMU is a peer-reviewed biomedical journal,published in Chinese with English abstracts.The journal aims to showcase outstanding research articles from all areas of biology and medicine,including basic medicine(such as biochemistry, microbiology, molecular biology, genetics, etc.),clinical medicine,public health and epidemiology, military medicine,pharmacology and Traditional Chinese Medicine),to publish significant case report, and to provide both perspectives on personal experiences in medicine and reviews of the current state of biology and medicine.