In vitro Experiments Revealed the Anthelmintic Potential of Herbal Complex against Haemonchus contortus

Abstract

Received: Revised: Accepted: Published online: October 8, 2019 December 9, 2019 December 24, 2019 December 31, 2019 In an era of drug resistance, new medicinal plants are being evaluated for control of parasites. In the present study, the anthelmintic activity of the extract of herbal complex (HC) composed of four herbs (Origanum vulgare, Cinnamomum verum, Rosmarinus officinalis and Capsicum annuum) was assessed against eggs and adult Haemonchus (H.) contortus. Egg hatch test (EHT) and adult motility assay (AMA) was performed to evaluate the anthelmintic potential of HC. The reference drug used in EHT was oxfendazole and for AMA two drugs i.e. oxfendazole (2.265 mg ml) and levamisole (3mg ml) were used. In EHT, LC50 values of HC and oxfendazole were 498 and 1.6 ppm, respectively. In AMA, 100% mortality of H. contortus was observed after 6 hr of treatment with HC (100 mg ml) where as two positive control groups could not kill all worms after this exposure time. These results indicated the anthelmintic potential of HC. ©2019 PVJ. All rights reserved