Brassinosteroid Biosynthetic Gene CmDWF4 Regulates Bud Outgrowth in Chrysanthemum morifolium

IF 0.1 4区生物学 Q4 PLANT SCIENCES Phyton-Annales Rei Botanicae Pub Date : 2023-01-01 DOI:10.32604/phyton.2023.027870

Xianrong Fu, A. Song, Bo Peng, Song Li, Weixin Liu, Lingling Zhang, Jiafu Jiang, Sumei Chen, Fadi Chen

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Abstract

Brassinosteroids (BRs), a class of steroid phytohormones, play a critical role in plant growth and development. The DWF4 gene encodes a cytochrome P450 enzyme (CYP90B1), which is considered a rate-limiting enzyme in BR biosynthesis. Here, we identi ﬁ ed a homologous gene of DWF4 in chrysanthemum, CmDWF4 . This gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point (pI) of 9.10. Overexpression of CmDWF4 in chrysanthemum was found to signi ﬁ cantly increase growth rate, number, and length of lateral buds. Transcriptome analysis showed that multiple xyloglucan endotransglycosy-lase/hydrolase (XTH) family encoding genes associated with cell wall modi ﬁ cation were up-regulated in CmDWF4 -overexpressing lines. qRT-PCR assay con ﬁ rmed the up-regulation of CmXTH6 , CmXTH23 , and CmXTH28 in CmDWF4 -overexpression line. Overall, this work establishes a mechanism by which BR biosynthetic gene CmDWF4 promotes lateral bud outgrowth in chrysanthemum, possibly through regulating cell elongation and expansion

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油菜素内酯生物合成基因CmDWF4调控菊花芽生长

油菜素内酯(BRs)是一类甾类植物激素，在植物生长发育中起着至关重要的作用。DWF4基因编码细胞色素P450酶(CYP90B1)，该酶被认为是BR生物合成中的限速酶。本研究在菊花中鉴定出DWF4的同源基因CmDWF4。该基因编码491个氨基酸残基，分子量为56.2 kDa，等电点(pI)为9.10。在菊花中过表达CmDWF4可以显著提高侧芽的生长速度、数量和长度。转录组分析显示，在CmDWF4过表达细胞系中，与细胞壁修饰相关的多个木葡聚糖内转糖酶/水解酶(XTH)家族编码基因上调。qRT-PCR检测证实CmXTH6、CmXTH23和CmXTH28在CmDWF4过表达系中表达上调。总之，本研究建立了BR生物合成基因CmDWF4促进菊花侧芽生长的机制，可能通过调控细胞伸长和扩增来实现

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Phyton-Annales Rei Botanicae 生物-植物科学

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