{"title":"DNA-sequence-based species delimitation for larval chironomid samples collected from Japanese farm ponds","authors":"K. Takamura, R. Ueno, N. Kondo, Kako Ohbayashi","doi":"10.3739/RIKUSUI.78.35","DOIUrl":null,"url":null,"abstract":"DNA-sequence-based species delimitation methods were applied to larval chironomid samples collected from 20 Japanese farm ponds. This is the first trial using DNA barcoding to reveal the species composition of chironomid communites in limnological waters. A total of 72 species were delimited from mitochondria DNA cytochrome c oxidase I sequences of the samples. Of these, the number of species delimited from the total sample, bottom sediment sample, and aquatic weed sample of each pond, were 0-21, 0-15 and 3-16, respectively. Greater numbers of collected larvae resulted in more delimited species. Scientific names were assigned for 31 species.","PeriodicalId":35602,"journal":{"name":"Japanese Journal of Limnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3739/RIKUSUI.78.35","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Limnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3739/RIKUSUI.78.35","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 1
Abstract
DNA-sequence-based species delimitation methods were applied to larval chironomid samples collected from 20 Japanese farm ponds. This is the first trial using DNA barcoding to reveal the species composition of chironomid communites in limnological waters. A total of 72 species were delimited from mitochondria DNA cytochrome c oxidase I sequences of the samples. Of these, the number of species delimited from the total sample, bottom sediment sample, and aquatic weed sample of each pond, were 0-21, 0-15 and 3-16, respectively. Greater numbers of collected larvae resulted in more delimited species. Scientific names were assigned for 31 species.
期刊介绍：
The Japanese Society of Limnology was founded in 1931 for the purpose of promoting and fostering the study of limnology in the broad meaning of the term. It publishes quarterly "The Japanese Journal of Limnology" and holds an annual meeting consisting of lectures, discussions and symposiums on limnological subjects.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
