{"title":"Location and osteogenesis of bone marrow stromal cells after implantation in vivo","authors":"董世武, 应大君, 糜建红, 刘光久, 王廷华, 羊惠君","doi":"10.3760/CMA.J.ISSN.1671-7600.2004.10.024","DOIUrl":null,"url":null,"abstract":"目的 探讨骨髓基质干细胞(BMSCs)与支架材料复合,并种植到动物体内后的定位及成骨情况.方法分离培养绿色荧光蛋白(GFP)小鼠BMSCs,体外扩增后与猪脱细胞骨基质材料复合,植入裸鼠背部作为实验组,并以单纯材料植入作为对照组,8周后进行大体观察、常规组织学检查及荧光检测.结果实验组移植物周围部成骨明显,GFP阳性细胞较多;中央部材料降解吸收,GFP阳性细胞少见.对照组成骨较少.结论BMSCs作为种子细胞参与了骨重建,同时GFP示踪是观测BMSCs的一种较好方法。","PeriodicalId":10145,"journal":{"name":"中华创伤骨科杂志","volume":"6 1","pages":"1164-1166"},"PeriodicalIF":0.0000,"publicationDate":"2004-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华创伤骨科杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.1671-7600.2004.10.024","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
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