The Sympathetic Nervous System Regulates Inflammation in Amoebic Liver Abscess in Hamsters

Abstract

An inflammation is generated during formation of Amoebic Liver Abscess. We analyzed chemically sympathectomized hamsters with Amoebic Liver Abscess, in a period between 6 hours to 7 days. The liver tissue samples were analyzed by haematoxylin and eosin staining, immunohistochemistry, immunofluorescence and morphometry. Sympathectomy caused a lower production of collagen and absence of granuloma tissue of Amoebic Liver Abscess. At the 6 h of development, proinflammatory cytokines were reduced, also reduced 12 hours – 7 days; while IL-10 production was incresed for this time; Transforming Growth Factorproducing cells only increased from 4 to 7 days. Nuclear factor kappa-light-chain-enhancer of activated B cells were diminished throughout the Amoebic Liver Abscess, while Toll Like Receptor 4 + macrophages decreased in the period of 2 to 7 days; and Toll Like Receptor 4 + neutrophils decreased between 4 and 7 days. The population of trophozoites was increased in sympathectomized animals between 4 and 7 days. The chemical sympathectomy reduces the collagen deposition and induces an anti-inflammatory state during the development of Amoebic Liver Abscess, then; allows the spread and reproduction of Entamoeba histolytica trophozoites.