Assessment of phylogenetic diversity and relationships among Grewia tenax population in Sudan using RAPD and ISSR molecular markers

IF 0.7 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY AIMS Molecular Science Pub Date : 2022-01-01 DOI:10.3934/molsci.2022001

R. S. Habeballa, Mutasim Mohamed Khalafalla Ali, R. S. Modawi

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Abstract

This research is conducted to study the genetic diversity of the genus Grewia tenax in Sudan. This plant has a common vernacular Sudanese Arabic name: “Gudeim”. It is mainly cultivated in North Darfur and Western Sudan. Common uses of Grewia species were overviewed in areas of nutrition, folk medicine and famine food. The genetic variation and genetic relationships among Grewia spp populations from different regions were efficiently determined using RAPD and ISSR markers.

The results of genetic analysis were statistically analyzed by STATISTCA and GenALEx 6.5 software. The results of molecular variance revealed that 74% of total genetic diversity was due to within populations variations as opposed to 26% due to variations between populations unweighted pair- group method with arithmetic average (UPGMA) were constructed for and RAPD + ISSR. The UPGMA results showed variability of Grewia genotypes.

It was concluded that both the marker systems RAPD and ISSR combination can be effectively used in determination of genetic relationships among Grewia tenax genotypes.

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利用RAPD和ISSR分子标记评价苏丹褐豆种群的系统发育多样性及亲缘关系

本研究是为了研究苏丹绿叶属植物的遗传多样性。这种植物有一个常见的苏丹阿拉伯方言名称:“Gudeim”。它主要种植在北达尔富尔和苏丹西部。在营养、民间医药和饥荒食品等领域概述了绿尾藤的常见用途。利用RAPD和ISSR标记，对不同地区绿芽甘蓝居群间的遗传变异和亲缘关系进行了分析。遗传分析结果采用STATISTCA和GenALEx 6.5软件进行统计学分析。分子变异分析结果表明，总遗传多样性的74%来自群体内变异，而26%来自群体间变异。UPGMA结果显示了Grewia基因型的变异性。结果表明，RAPD和ISSR组合标记系统均可有效地用于黄颡鱼基因型间的亲缘关系测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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AIMS Molecular Science BIOCHEMISTRY & MOLECULAR BIOLOGY-

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