S. Ramzi, A. Sahragard, Jalal Jalali Sendi, A. Aalami
{"title":"Effect of Citrullus colocynthis L. (Cucurbitaceae) agglutinin on gene expression of caspases in Ectomyelois ceratoniae Zeller (Lepidoptera: Crambidae)","authors":"S. Ramzi, A. Sahragard, Jalal Jalali Sendi, A. Aalami","doi":"10.4081/JEAR.2016.5699","DOIUrl":null,"url":null,"abstract":"The larvae of Ectomyelois ceratoniae Zeller were reared on the artificial diets containing 2% of Citrullus colocynthis L. (Cucurbitaceae) lectin and control to find any changes in gene expression of caspases. Specific primers were designed to amplify genes of six found caspases including Caspase 1, 2, 3, 4, 5 and 6. RNA of both treatments were extracted and cDNA molecules were synthesized prior to gene expression. Results of quantitative real-time polymerase chain reaction revealed that expression of all caspases in the fed larvae by 2% of lectin increased in comparison with control except for caspase 1 as 2.99, 5.7, 17.13, 23.18, 10.44-fold. These results are consistent with our previous studies on the lower activities of digestive enzymes that are due to death of midgut cells followed by binding of lectin molecule.","PeriodicalId":37777,"journal":{"name":"Journal of Entomological and Acarological Research","volume":"48 1","pages":"304-307"},"PeriodicalIF":0.0000,"publicationDate":"2016-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JEAR.2016.5699","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Entomological and Acarological Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4081/JEAR.2016.5699","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1
Abstract
The larvae of Ectomyelois ceratoniae Zeller were reared on the artificial diets containing 2% of Citrullus colocynthis L. (Cucurbitaceae) lectin and control to find any changes in gene expression of caspases. Specific primers were designed to amplify genes of six found caspases including Caspase 1, 2, 3, 4, 5 and 6. RNA of both treatments were extracted and cDNA molecules were synthesized prior to gene expression. Results of quantitative real-time polymerase chain reaction revealed that expression of all caspases in the fed larvae by 2% of lectin increased in comparison with control except for caspase 1 as 2.99, 5.7, 17.13, 23.18, 10.44-fold. These results are consistent with our previous studies on the lower activities of digestive enzymes that are due to death of midgut cells followed by binding of lectin molecule.
期刊介绍：
The Journal of Entomological and Acarological Research (JEAR), formerly the Bollettino di Zoologia Agraria e di Bachicoltura of the Institute of Entomology of the Università degli Studi, Milano, was founded in 1928 by Remo Grandori. Thereafter, Minos Martelli and Luciano Süss hold the direction of the Journal until December 2011. In January 2012 the Editor decided for the new open-access on-line version of JEAR. The Journal publishes original research papers concerning Arthopods, but reviews, editorials, technical reports, brief notes, conference proceeding, letters to the Editor, book reviews are also welcome. JEAR has four main areas of interest: -Entomology (systematics; morphology; biology; biotechnology; agriculture, ornamental and forest entomology; applied entomology; integrated pest management; biological control; apiculture and apidology; medical, urban and veterinary entomology; etc.) -Stored product pests (biology; integrated pest management; etc.) -Insect Ecology (behaviour; biodiversity; taxonomy; plant insect interaction and ecosystems; biological control; alien species; etc.) -Acarology (systematics; morphology; biology; parasitology; control; etc.) The publication of manuscripts is subject to the approval of the Section Editor who has knowledge of the field discussed in the manuscript in accordance with the principles of Peer Review; referees will be selected from the Editorial Board or among qualified scientists of the international scientific community. Articles must be written in English and must adhere to the guidelines and details contained in the Instructions to Authors.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
