The In vitro and In vivo Efficacy of Gold Nanoparticle in Comparison to the Glucantime as a Therapeutic Agent against L. major

Abstract

To evaluate the anti-leishmanial activity of gold nanoparticle against Iranian strain of Leishmania major (MRHo/IR/75/ER): In vitro and In vivo study. Gold nanoparticle was prepared by heating 20 ml of HAuCl4 (1.0 Mm) on a moving hot plate and concentrations of 100, 500 and 1000 ppm. parasites were added in different wells and 20 μL of each concentration were added in medium. Live and dead Promastigotes were counted after adding 0.1% eosin stain. Moreover, the efficacy of gold nanoparticle was examined in BALB/c mice infected with Iranian strain of L. major. The gold nanoparticle had completely effective at concentration of 500 and 1000 ppm on promastigote from L. major after 180 min. In In vivo study, the mean size of lesions was significantly decreased in the groups treated with gold nanoparticle in comparison with the control group. Gold nanoparticle was predictable as an appropriate candidate among natural antileishmanial agents.