Yaoyao Liu, Miao-miao Sun, Qinru Yu, Ran Li, Xueping Hu, Pengcheng Zhang, T. Lai, Ting Zhou
{"title":"Identification and Analysis of miRNAs and siRNAs in <i>Botrytis cinerea</i>","authors":"Yaoyao Liu, Miao-miao Sun, Qinru Yu, Ran Li, Xueping Hu, Pengcheng Zhang, T. Lai, Ting Zhou","doi":"10.4236/ajps.2022.135042","DOIUrl":null,"url":null,"abstract":"Small RNAs in Botrytis cinerea were analyzed via high-throughput sequencing on BGISEQ-500 platform. A total of 8 novel miRNAs and 110 novel siRNAs were predicted. Sequence information, construction, length distribution, base bias and expression levels of miRNAs and siRNAs were determined as well. Through GO and KEGG enrichment analysis, the miRNA target genes are mostly located in membrane and organelle, possessed binding and catalytic activities, and involved in signal transduction and carbohydrate metabolism. The results will provide a theoretical foundation for understanding the developmental and pathogenic mechanisms of B. cinerea at the transcriptional level.","PeriodicalId":56643,"journal":{"name":"美国植物学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"美国植物学期刊(英文)","FirstCategoryId":"1091","ListUrlMain":"https://doi.org/10.4236/ajps.2022.135042","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Small RNAs in Botrytis cinerea were analyzed via high-throughput sequencing on BGISEQ-500 platform. A total of 8 novel miRNAs and 110 novel siRNAs were predicted. Sequence information, construction, length distribution, base bias and expression levels of miRNAs and siRNAs were determined as well. Through GO and KEGG enrichment analysis, the miRNA target genes are mostly located in membrane and organelle, possessed binding and catalytic activities, and involved in signal transduction and carbohydrate metabolism. The results will provide a theoretical foundation for understanding the developmental and pathogenic mechanisms of B. cinerea at the transcriptional level.
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