SEROLOGICAL FEATURES OF ANTIBODIES TO COMBINED ABH-LEWIS ANTIGENS PRODUCED IN A PATIENT AFTER OPEN-HEART SURGICAL OPERATION

Abstract

SEROLOGICAL FEATURES OF ANTIBODIES TO COMBINED ABH-LEWIS ANTIGENS PRODUCED IN A PATIENT AFTER OPEN-HEART SURGICAL OPERATION Hiroyasu Yasuda, Hitoshi Ohto, Kazuko Hirahara, Yuriko Tohyama, Tetsunori Tasaki, Ryoichi Motokil, Satoru Arai2 and Tsuneyoshi Saito3 Blood Transfusion Service, Fukushima Medical College 2Department of Cardiac Surgery, Tohoku Kousai Hospital 3Department of Internal Medicine, Yonezawa Municipal Hospital We found antibodies seropositive for lymphocytoxicity test (LCT) in a 60-year-old male patient, underwent an aorto-coronary bypass surgery for angina pectoris. He had received 800 ml of autologous blood, 940 ml of 25% human albumin and 720 mg of fibrin glue. Anti-human leukocyte antigen (HLA) antibody is frequently produced as a result of alloimmunization induced by homologous blood transfusions and even infusion of human plasma preparations including soluble HLA antigens. LCT reactive antibodies produced in the patient were proved to be not against HLA, as they were not absorbed by pooled platelets from type 0 blood group multiple donors, nor reacted with any of 18 B-cell lines. The antibodies reacted only with panel T-cells from A Led, B Led donors. These antibodies identified were anti-A and -B, mainly combined ABHLewis antigens, as the results demonstrated by lymphocytotoxicity test using the patient's sera after absorption with homologous red blood cells, lymphocytes, platelets, and with columns coated by A, B and Lewis substances. The antibodies seem to be induced by some human plasma preparations with soluble ABO blood group antigens infused during perioperative period, although, details of the mechanism remain obscure.