Developing cell-free protein synthesis systems: a focus on mammalian cells

A. K. Brödel, S. Kubick
{"title":"Developing cell-free protein synthesis systems: a focus on mammalian cells","authors":"A. K. Brödel, S. Kubick","doi":"10.4155/PBP.14.30","DOIUrl":null,"url":null,"abstract":"Sophisticated cell-free protein synthesis (CFPS) systems have been developed as an alternative to recombinant expression in cultured cells. In this review, we present advances in the field of mammalian-based CFPS by highlighting recently established systems derived from mouse fibroblasts, HeLa, hybridoma, CHO and K562 cells. We further highlight ongoing challenges in the field of mammalian-based CFPS, such as the optimization of already established platforms and the development of novel systems in order to further increase protein yields and reduce manufacturing costs while facilitating the synthesis of a huge number of biologically active target proteins. Advances in mammalian-based CFPS shall expand the number of future applications of CFPS in the area of pharmaceutical research and development.","PeriodicalId":90285,"journal":{"name":"Pharmaceutical bioprocessing","volume":"101 1","pages":"339-348"},"PeriodicalIF":0.0000,"publicationDate":"2014-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4155/PBP.14.30","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceutical bioprocessing","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4155/PBP.14.30","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11

Abstract

Sophisticated cell-free protein synthesis (CFPS) systems have been developed as an alternative to recombinant expression in cultured cells. In this review, we present advances in the field of mammalian-based CFPS by highlighting recently established systems derived from mouse fibroblasts, HeLa, hybridoma, CHO and K562 cells. We further highlight ongoing challenges in the field of mammalian-based CFPS, such as the optimization of already established platforms and the development of novel systems in order to further increase protein yields and reduce manufacturing costs while facilitating the synthesis of a huge number of biologically active target proteins. Advances in mammalian-based CFPS shall expand the number of future applications of CFPS in the area of pharmaceutical research and development.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
开发无细胞蛋白质合成系统:以哺乳动物细胞为重点
复杂的无细胞蛋白合成(CFPS)系统已经发展成为在培养细胞中重组表达的替代方法。在这篇综述中,我们介绍了基于哺乳动物的CFPS领域的进展,重点介绍了最近建立的来自小鼠成纤维细胞、HeLa、杂交瘤、CHO和K562细胞的系统。我们进一步强调了基于哺乳动物的CFPS领域正在面临的挑战,例如优化已经建立的平台和开发新的系统,以进一步提高蛋白质产量和降低制造成本,同时促进大量生物活性靶蛋白的合成。基于哺乳动物的CFPS的进展将扩大CFPS在药物研发领域的未来应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Bioresources and Bioprocessing a review Short Commentary on Pharmaceutical Bioprocessing Editorial in Pharmaceutical Bioprocessing Dynamics and scale-up of thePharmaceutical molecule Research on Pharmaceutical Bioprocessing a short communication
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1