Recent advances in optimal cell banking of mammalian cells for biopharmaceutical production

Abstract

Mammalian cells are routinely used in the biopharmaceutical industry for production of recombinant therapeutic proteins. Cell banking to ensure preservation of these cells at low temperatures for an extended period of time is at the core of establishing a manufacturing process utilizing these cells and various strategies have evolved over time to ensure recovery of viable ‘and’ functional cells. This paper provides an overview of cryopreservation practices and highlights recent approaches that have been adopted to improve cryopreservation outcomes for these industrially relevant production cell lines.