{"title":"Caveolin-1 Inhibits Proliferation and Migration of Gastric Cancer Cell via Inactivating BMI-1","authors":"Jun Wang, Shasha Liu","doi":"10.4236/ojpathology.2022.123010","DOIUrl":null,"url":null,"abstract":"Objective: To investigate the effect of Caveolin-1on the proliferation and migration ability in gastric carcinoma cells MGC-803 and its mechanism. Methods: Plasmid DNA pcDNA3.1-Cav1 was extracted by extracted kits, and transfected Cav-1 gene sequences were found. The expression levels of Cav-1 protein were detected by Western blot. And the proliferation was analyzed by CCK8 assay. The effect of Cav-1 on migration was detected by wound healing. The expression levels of BMI-1 protein were detected by western blot. Results: 1) Western Blot showed that the expression levels of Cav-1 were higher in MGC-803/Cav-1 than control group, P < 0.05. 2) CCK8 showed, that the proliferation of MGC-803/Cav-1 was inhibited, but P > 0.05. Wound healing showed, that the migration ability of MGC-803/Cav-1 fell off, P < 0.05. 3) Western Blot showed that the expression levels of BMI-1 were lower in MGC-803/Cav-1 than in the control group, P < 0.05. Conclusion: Cave-olin-1 can inhibit the proliferation and migration ability of gastric carcinoma cells and its mechanism may relate to BMI-1.","PeriodicalId":57444,"journal":{"name":"病理学期刊(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"病理学期刊(英文)","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4236/ojpathology.2022.123010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To investigate the effect of Caveolin-1on the proliferation and migration ability in gastric carcinoma cells MGC-803 and its mechanism. Methods: Plasmid DNA pcDNA3.1-Cav1 was extracted by extracted kits, and transfected Cav-1 gene sequences were found. The expression levels of Cav-1 protein were detected by Western blot. And the proliferation was analyzed by CCK8 assay. The effect of Cav-1 on migration was detected by wound healing. The expression levels of BMI-1 protein were detected by western blot. Results: 1) Western Blot showed that the expression levels of Cav-1 were higher in MGC-803/Cav-1 than control group, P < 0.05. 2) CCK8 showed, that the proliferation of MGC-803/Cav-1 was inhibited, but P > 0.05. Wound healing showed, that the migration ability of MGC-803/Cav-1 fell off, P < 0.05. 3) Western Blot showed that the expression levels of BMI-1 were lower in MGC-803/Cav-1 than in the control group, P < 0.05. Conclusion: Cave-olin-1 can inhibit the proliferation and migration ability of gastric carcinoma cells and its mechanism may relate to BMI-1.
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