Raman Spectroscopy for Forensic Identification of Body Fluid Traces: Method Validation for Potential False Negatives Caused by Blood-Affecting Diseases

Niara A. Nichols, I. Lednev
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引用次数: 4

Abstract

Two critical issues in forensic science are identifying body fluid traces found at crime scenes and preserving them for DNA analysis. However, the majority of current biochemical tests for body fluid identification, which are applicable at the crime scene, are presumptive and destructive to the sample. Raman Spectroscopy provides a suitable alternative to current methods as a nonde-structive, confirmatory, and potentially in field method. Our laboratory has developed a chemometric model for the identification of five main body fluids using Raman spectroscopy. This model was developed using samples obtained from healthy donors. Thus, it is of most importance for the forensic application of the method to validate its performance for donors with diseases that might affect the biochemical composition of body fluids. In this study, the developed method was validated using peripheral blood samples acquired from donors with Celiac Disease, Sickle Cell Anemia, and Type 2 Diabetes. It was shown that the method correctly identified all samples as peripheral blood indicating that no false positives could occur because the blood traces were originated from donors suffering from the diseases.
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拉曼光谱法鉴定体液痕迹:影响血液的疾病引起的潜在假阴性的方法验证
法医科学的两个关键问题是鉴定在犯罪现场发现的体液痕迹和保存它们以供DNA分析。然而,目前大多数适用于犯罪现场的体液鉴定生化试验都是假定的,对样本具有破坏性。拉曼光谱作为一种非破坏性、验证性和潜在的现场方法,为当前的方法提供了合适的替代方法。我们的实验室开发了一种化学计量模型,用于使用拉曼光谱识别五种主要体液。该模型是使用从健康供体获得的样本开发的。因此,对于该方法的法医应用来说,最重要的是验证其对可能影响体液生化组成的疾病供体的性能。在这项研究中,使用乳糜泻、镰状细胞性贫血和2型糖尿病患者的外周血样本验证了所开发的方法。结果表明,该方法正确地将所有样本识别为外周血,表明不会出现假阳性,因为血液痕迹来自患有这些疾病的献血者。
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