Simultaneous Determination by HPLC-UV Vis of Tartrazine and Sunset Yellow in Soft Drinks Sold in Benin

Janvier Engelbert Agbokponto, A. P. S. Kpaibé, L. Yemoa, A. G. Assanhou, H. Ganfon, G. Gbassi, M. Ake, N’cho Christoph Amin
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引用次数: 4

Abstract

The use of dyes such as tartrazine (E102) and sunset yellow (E102) in food, beverages and health products for technological and commercial purposes is common. The adverse effects caused by these dyes, such as allergies and hyperactivity disorder have been reported, especially in children. In the present study, a chromatographic method was developed and validated for simultaneous determination of tartrazine and sunset yellow. The chromatographic separation was performed on a Lichrocart ® C18 column (125 × 4.6 mm; 5 µm) with a security Guard-C 18 column (4 × 2.0 mm, 5 µm; Phenomenex, Torrance, CA, USA) maintained at 30˚C. The mobile tartrazine and sunset yellow in soft drinks sold in Benin.
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HPLC-UV - Vis同时测定贝宁市售软饮料中的酒黄石和日落黄
出于技术和商业目的,在食品、饮料和保健产品中使用酒黄石(E102)和日落黄(E102)等染料是很常见的。这些染料引起的不良反应,如过敏和多动障碍,已被报道,特别是在儿童中。本研究建立了同时测定酒黄石和日落黄的色谱方法。色谱分离采用Lichrocart®C18色谱柱(125 × 4.6 mm;5µm)与security Guard-C 18柱(4 × 2.0 mm, 5µm;Phenomenex, Torrance, CA, USA)保持在30˚C。在贝宁销售的软饮料中含有流动酒黄石和日落黄。
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