Application of DPPH assay for the evaluation of the antiradical activity of Creatine Lysinate

Abstract

The aim of the current study was the estimation of the radical-scavenging activity of Creatine lysinate against 0.05 mM methanol solution of DPPH radical by measuring the decrease in the absorbance at λ = 516 in methanol. The antiradical effect of the compound examined is presented with the following parameters calculated: radical scavenging activity in [%], IC 50 value; antioxidant power 1/IC 50 , and Trolox equivalent activity. relative radical scavenging activity (RRSA, [%]), and a relative decrease in radical scavenging activity (RDRSA, [%]).From the experimental results, it was observed that the DPPH binding ability of Creatine lysinate (IC 50 = 73.75 mM) is lower compared to the standard Trolox (IC 50 = 0.001154 mM), which antioxidant power (1/IC 50 = 8.67) is higher in comparison with Creatine lysinate (1/IC 50 = 0.014). The experimental data show that Creatine lysinate (IC 50 = 73.75 mM) is more active compared to Creatine monohydrate (IC 50 = 102.48 mM) due to lower IC 50 and higher scavenging activity. In comparison with mono application of alone antioxidant, the beneficial effect of the combination of Creatine monohydrate and Creatine lysinate with other antioxidants in form of food supplements could be an important strategy for a synergistic effect in the reduction of free radicals in the treatment of disorders resulted from oxidative stress.