Research Article Virulence genes in Staphylococcus aureus isolated from cell phone screens of dentistry students in Cuenca-Ecuador

IF 0.6 Q4 GENETICS & HEREDITY Genetics and Molecular Research Pub Date : 2021-01-01 DOI:10.4238/gmr18928

M. Pacheco, P. P. Orellana, C. Andrade, J.E. Torracchi

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Abstract

Staphylococcus aureus is one of the main pathogens of importance in health care. Distributed worldwide, it has considerable impact on community and nosocomial infections. This bacteria has the tst and lukS-F PV genes that code for the TSST-1 toxin and leucocidin. These pathogenic microorganisms have the ability to survive for long periods on inert surfaces such as mobile phone screens. Senior dentistry students work in clinics and use their cell phones often, which could potentially spread this pathogen. We looked for the tst and lukS-F PV genes in S. aureus isolated from mobile phone screens of final year dentistry students. This was a descriptive cross-sectional observational study using 92 samples from the mobile phone screens of dental students, among which 16 were positive for S. aureus. They were identified by means of culture methods and detection of the nuc, nucA and femB genes. The DNA was extracted using the alkaline lysis method; PCR was used in the molecular identification of the tst and lukS-F PV genes. Nine of the 16 S. aureus isolates had the tst gene, and 1/16 the lukS-F PV gene. This study shows the high frequency of virulent S. aureus on the

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从厄瓜多尔昆卡牙科学生手机屏幕中分离的金黄色葡萄球菌的毒力基因

金黄色葡萄球菌是卫生保健中重要的主要病原体之一。它分布在世界各地，对社区和医院感染有相当大的影响。这种细菌具有编码TSST-1毒素和杀白细胞素的tst和lukS-F PV基因。这些致病微生物有能力在手机屏幕等惰性表面长时间存活。牙科专业的高年级学生在诊所工作，经常使用手机，这可能会传播这种病原体。我们从牙科学生的手机屏幕中分离出金黄色葡萄球菌，寻找测试和lukS-F PV基因。这是一项描述性横断面观察性研究，使用了来自牙科学生手机屏幕的92个样本，其中16个对金黄色葡萄球菌呈阳性。通过培养法和nuc、nucA和femB基因的检测，对它们进行了鉴定。采用碱法提取DNA;采用PCR方法对tst和lukS-F PV基因进行分子鉴定。16株金黄色葡萄球菌分离株中有9株含有test基因，1/16含有lukS-F PV基因。本研究表明，金黄色葡萄球菌在大肠杆菌上的高致病性

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来源期刊

Genetics and Molecular Research 生物-生化与分子生物学

CiteScore

1.00

自引率

25.00%

发文量

审稿时长

3 months

期刊介绍： Genetics and Molecular Research (GMR), maintained by the Research Foundation of Ribeirão Preto (Fundação de Pesquisas Científicas de Ribeirão Preto), publishes high quality research in genetics and molecular biology. GMR reflects the full breadth and interdisciplinary nature of this research by publishing outstanding original contributions in all areas of biology. GMR publishes human studies, as well as research on model organisms—from mice and flies, to plants and bacteria. Our emphasis is on studies of broad interest that provide significant insight into a biological process or processes. Topics include, but are not limited to gene discovery and function, population genetics, evolution, genome projects, comparative and functional genomics, molecular analysis of simple and complex genetic traits, cancer genetics, medical genetics, disease biology, agricultural genomics, developmental genetics, regulatory variation in gene expression, pharmacological genomics, evolution, gene expression, chromosome biology, and epigenetics.