Characterization of Coolia spp. (Gonyaucales, Dinophyceae) from Southern Tunisia: first record of Coolia malayensis in the Mediterranean Sea

IF 3.1 3区生物学 Q1 MARINE & FRESHWATER BIOLOGY Algae Pub Date : 2021-09-15 DOI:10.4490/algae.2021.36.6.2

Moufida Abdennadher, A. Zouari, W. Medhioub, A. Penna, A. Hamza

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引用次数: 4

Abstract

This study provides the first report of the presence of Coolia malayensis in the Mediterranean Sea, co-occurring with C. monotis. Isolated strains from the Gulf of Gabès, Tunisia (South-eastern Mediterranean) were identified by morphological characterization and phylogenetic analysis. Examination by light and scanning electron microscopy revealed no significant morphological differences between the Tunisian isolates and other geographically distant strains of C. monotis and C. malayensis. Phylogenetic trees based on ITS1-5.8S-ITS2 and D1‒D3/28S rDNA sequences showed that C. monotis strains clustered with others from the Mediterranean and Atlantic whereas the C. malayensis isolate branched with isolates from the Pacific and the Atlantic, therefore revealing no geographical trend among C. monotis and C. malayensis populations. Ultrastructural analyses by transmission electron microscopy revealed the presence of numerous vesicles containing spirally coiled ﬁbers in both C. malayensis and C. monotis cells, which we speculate to be involved in mucus production.

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突尼斯南部Coolia属(Gonyaucales, Dinophyceae)的特征:地中海首次记录的马来西亚Coolia

本研究首次报道了马来Coolia在地中海的存在，与C. monotis共存。通过形态鉴定和系统发育分析，对来自突尼斯gab湾(地中海东南部)的分离菌株进行了鉴定。光镜和扫描电镜检查显示，突尼斯分离株与其他地理距离较远的C. monotis和C. malayensis菌株在形态上没有显著差异。基于ITS1-5.8S-ITS2和D1-D3/28S rDNA序列的系统进化树显示，单孢梭菌与来自地中海和大西洋的其他菌株聚集在一起，而马来梭菌分离株与来自太平洋和大西洋的分离株形成分支，因此单孢梭菌和马来梭菌之间没有地理趋势。透射电子显微镜的超微结构分析显示，C. malayensis和C. monotis细胞中存在大量含有螺旋卷曲纤维的囊泡，我们推测这些囊泡参与了粘液的产生。

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来源期刊

Algae PLANT SCIENCES-

CiteScore

5.10

自引率

25.00%

发文量

期刊介绍： ALGAE is published by the Korean Society of Phycology and provides prompt publication of original works on phycology. ALGAE publishes articles on all aspects of phylogenetics and taxonomy, ecology and population biology, physiology and biochemistry, cell and molecular biology, and biotechnology and applied phycology. Checklists or equivalent manu-scripts may be considered for publication only if they contribute original information on taxonomy (e.g., new combinations), ecology or biogeography of more than just local relevance. Contributions may take the form of Original Research Articles, Research Notes, Review Articles and Book Reviews.