Fractal analysis of flavonoids in complex chemical compositions in extracts of Chamaedaphne calyculata (L.) Moench (ericaceae) in oligotrophic swamps of Western Siberia

Abstract

Evaluated the possibility of organizing the biosynthesis of plant adaptogens-flavonoids from the standpoint of the theory of neutrality. We assumed that the biosynthesis of flavonoids and low-molecular compounds with similar physicochemical properties is neutral with respect to microfluctuations of environmental conditions. Leaf extracts Chamaedaphne calyculata (L.) Moench from natural populations oligotrophic swamps the Middle Ob region (Western Siberia) was studied by method HPLC-high performance liquid chromatography. The samples to carry out the HPLC analyses were taken in ecologically equivalent swamp areas. Chromatograms differed in the number of peaks, the time of their release, and the area of particular peaks. The hierarchy constructing procedure from single up to "combined" chromatograms, where peaks from 1, 2, 3, 5, and 9 chromatograms were placed on the common time scale of the peaks release, was carried out for fractal analysis. The number of peaks varied from 21-30 in particular chromatograms up to 76 peaks in a "generalized" united chromatogram. The complex of calculation procedures has shown that all chromatograms obtained from Chamaedaphne calyculata plants are stochastic fractals, where the independent variables are the number, the size (area) and release time of each particular peak. In the presence of the fractal properties of the flavonoid system, it is necessary to analyze the possibility of implementing both neutral and deterministic variants of the synthesis of this or that flavonoid.