Per-deuteration and NMR experiments for the backbone assignment of 62 kDa protein, Hsp31

Abstract

Hsp31 protein is one of the members of DJ-1 superfamily proteins and has a dimeric structure of which molecular weight (MW) is 62 kDa. The mutation of DJ-1 is closely related to early onset of Parkinson’s disease. Hsp31 displays Zn +2 -binding activity and was first reported to be a holding chaperone in E. coli . Its additional glyoxalase III active has recently been characterized. Moreover, an incubation at 60 ° C induces Hsp31 protein to form a high MW oligomer (HMW) in vitro , which accomplishes an elevated holding chaperone activity. The NMR technique is elegant method to probe any local or global structural change of a protein in responses to environmental stresses (heat, pH, and metal). Although the presence of the backbone chemical shifts (bbCSs) is a prerequisite for detailed NMR analyses of the structural changes, general HSQC-based triple resonance experiments could not be used for 62 kDa Hsp31 protein. Here, we prepared the per-deuterated Hsp31 and performed the TROSY-based triple resonance experiments for the bbCSs assignment. Here, detailed processes of per-deuteration and the NMR experiments are described for other similar NMR approaches.