{"title":"Effect of Moringa Leaf Extract (<i>Moringa oleifera</i> Lam.) on the Diameter of the Primary and Secondary Follicles in Female Mice (<i>Mus musculus</i>).","authors":"Amiruddin, Lili Darlian, Sriyana Herman, Mayang Sari, Rusli","doi":"10.3923/pjbs.2023.360.370","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background and Objective:</b> Infertility is still a phenomenon in the community, so consuming Moringa leaves (<i>Moringa oleifera</i> Lam.) is expected to increase fertility. This study aimed to determine the effect of Moringa leaf extract (<i>Moringa oleifera</i> Lam.) on the diameter of the primary and secondary follicles in female mice (<i>Mus musculus</i>). <b>Materials and Methods:</b> This study was an experiment using a Completely Randomized Design (CRD). The population of this study was 45 mice and samples were obtained by a simple random sampling technique from as many as 24 mice with the following criteria: Weight 20-25 g, 2-3 months old, female and in good health. Data analysis was performed through the ANOVA Test with a confidence level of α = 0.05 and further tested for the least significant difference (LSD). <b>Results:</b> Moringa leaf extract significantly positively affects the diameter of primary and secondary follicles in female mice (p<0.05). The average primary follicle diameter was P<sub>0</sub> (92.65 μm), P<sub>1</sub> (124.92 μm), P<sub>2 </sub>(150.72 μm), P<sub>3</sub> (175.68 μm) and the average secondary follicle diameter was control (157.17 μm), P<sub>1 </sub>(171.33 μm), P<sub>2</sub> (204.57 μm), P<sub>3</sub> (211.11 μm). Giving Moringa leaf extract (<i>Moringa oleifera</i> Lam.) significantly increases the diameter of mice's primary and secondary follicles due to the presence of vitamin E in Moringa leaf extract (<i>Moringa oleifera</i> Lam.). <b>Conclusion:</b> This can stimulate granulosa cells to secrete the hormone estrogen, causing an increase in the diameter of the primary and secondary follicles.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan Journal of Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3923/pjbs.2023.360.370","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
<b>Background and Objective:</b> Infertility is still a phenomenon in the community, so consuming Moringa leaves (<i>Moringa oleifera</i> Lam.) is expected to increase fertility. This study aimed to determine the effect of Moringa leaf extract (<i>Moringa oleifera</i> Lam.) on the diameter of the primary and secondary follicles in female mice (<i>Mus musculus</i>). <b>Materials and Methods:</b> This study was an experiment using a Completely Randomized Design (CRD). The population of this study was 45 mice and samples were obtained by a simple random sampling technique from as many as 24 mice with the following criteria: Weight 20-25 g, 2-3 months old, female and in good health. Data analysis was performed through the ANOVA Test with a confidence level of α = 0.05 and further tested for the least significant difference (LSD). <b>Results:</b> Moringa leaf extract significantly positively affects the diameter of primary and secondary follicles in female mice (p<0.05). The average primary follicle diameter was P<sub>0</sub> (92.65 μm), P<sub>1</sub> (124.92 μm), P<sub>2 </sub>(150.72 μm), P<sub>3</sub> (175.68 μm) and the average secondary follicle diameter was control (157.17 μm), P<sub>1 </sub>(171.33 μm), P<sub>2</sub> (204.57 μm), P<sub>3</sub> (211.11 μm). Giving Moringa leaf extract (<i>Moringa oleifera</i> Lam.) significantly increases the diameter of mice's primary and secondary follicles due to the presence of vitamin E in Moringa leaf extract (<i>Moringa oleifera</i> Lam.). <b>Conclusion:</b> This can stimulate granulosa cells to secrete the hormone estrogen, causing an increase in the diameter of the primary and secondary follicles.
期刊介绍:
Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.