Novel Polymorphisms in Caprine Myostatin Gene and its Relationship with Growth Traits in Saburai Does (Capra hircus).

Abstract

<b>Background and Objective:</b> Saburai goat (<i>Capra hircus</i>) is a crossbred goat from Boer buck (75%) and Ettawa doe (25%) for meat production purposes. This study was carried out to detect the mutation points or Single Nucleotide Polymorphisms (SNPs) in the myostatin (<i>MSTN</i>) gene of Saburai with the forward sequencing method. <b>Materials and Methods:</b> A total of twenty-one blood samples of Saburai does (75% Boer, 25% Ettawa) were collected from the Villager Breeder Center (VBC) at Tanggamus Regency of Lampung Province, Indonesia. The DNA analysis consists of DNA isolation, PCR analysis and sequencing analysis. The record data were used for association study with the mathematical model: Y_ij = μ+G_i+Є_ij. <b>Results:</b> Research showed that one common SNP of g.217_218.indel.TTTTA (5'UTR) and three novel SNPs of c.386G>C (exon 1), g.641_642.indel.T (intron 2) and c.4957G>C (exon 3) were detected in the present study. In this study, a novel SNP on exon 1 and intron 2 of Saburai <i>MSTN</i> gene has a moderate PIC value (>0.30). In addition, a novel SNP on exon 1 and exon 3 of the Saburai <i>MSTN</i> gene was detected as a missense mutation of A55P and A43P, respectively. Goats with the heterozygous genotype have higher growth traits compared to goats with the homozygous genotype. <b>Conclusion:</b> The goats with heterozygous genotypes can be further developed to increase the productivity of Saburai goats.