{"title":"Testicular Effect of Selenium Nanoparticles on Monosodium Glutamate Induced Alteration in Male Albino Rats.","authors":"Maha Saud Alrashidi, Heba Fawzy Gomaa","doi":"10.3923/pjbs.2023.347.359","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background and Objective:</b> The flavor enhancer Monosodium Glutamate (MSG) is mostly utilized in Asian and West African cuisines, especially in West African and Asian dishes. However, due to its availability, largely without labeling, in many food products, unintentional overuse of this food additive may occur. The objective of this study was to find out how selenium nanoparticles affected the toxicity of MSG in male albino rats' testicles. <b>Materials and Methods:</b> As 35 Wistar male rats partitioned into 5 groups: G1: Control rats, G2: Received Se-NPs at 0.4 mg kg<sup>1</sup> b.wt., orally, G3: Injected with MSG at a daily dose of 4 g kg<sup>1</sup> b.wt., intraperitoneally (IP), G4: Ingested a daily oral dose of Se-NPs for 7 successive days and on the 7th day, received the first dose of MSG IP 4 g kg<sup>1</sup> b.wt., then received both treatments till the end of the study and G5: Administered a daily oral dose of 4 g kg<sup>1</sup> MSG, followed by Se-NPs at a daily dose of 0.4 mg kg<sup>1</sup> b.wt., the experiment continued for 28 days. Serum testosterone hormone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), the levels of serum lipid peroxidation (MDA), reduced Glutathione (GSH), Glutathione Peroxidase (GSH-Px), superoxide dismutase (SOD) and Lactate Dehydrogenase (LDH) were estimated and samples from testis were separated for histological analysis. <b>Results:</b> The MSG treatment induced a significant decline in the values of serum testosterone, FSH, LH, GSH, GSH-Px and SOD. It also increased the values of serum MDA and LDH and spermatic arrest. While, the administration of Se-NPs orally before MSG treatment resulted in a decline in the values of serum MDA and LDH, an elevation in the values of serum GSH, GSH-PX and SOD, testosterone, FSH, LH and reappearance of sperm. <b>Conclusion:</b> The use of Se-NPs as a protector exhibited more improvement in values of estimated hormones and oxidative stress markers than using it as a therapy.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan Journal of Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3923/pjbs.2023.347.359","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
<b>Background and Objective:</b> The flavor enhancer Monosodium Glutamate (MSG) is mostly utilized in Asian and West African cuisines, especially in West African and Asian dishes. However, due to its availability, largely without labeling, in many food products, unintentional overuse of this food additive may occur. The objective of this study was to find out how selenium nanoparticles affected the toxicity of MSG in male albino rats' testicles. <b>Materials and Methods:</b> As 35 Wistar male rats partitioned into 5 groups: G1: Control rats, G2: Received Se-NPs at 0.4 mg kg<sup>1</sup> b.wt., orally, G3: Injected with MSG at a daily dose of 4 g kg<sup>1</sup> b.wt., intraperitoneally (IP), G4: Ingested a daily oral dose of Se-NPs for 7 successive days and on the 7th day, received the first dose of MSG IP 4 g kg<sup>1</sup> b.wt., then received both treatments till the end of the study and G5: Administered a daily oral dose of 4 g kg<sup>1</sup> MSG, followed by Se-NPs at a daily dose of 0.4 mg kg<sup>1</sup> b.wt., the experiment continued for 28 days. Serum testosterone hormone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), the levels of serum lipid peroxidation (MDA), reduced Glutathione (GSH), Glutathione Peroxidase (GSH-Px), superoxide dismutase (SOD) and Lactate Dehydrogenase (LDH) were estimated and samples from testis were separated for histological analysis. <b>Results:</b> The MSG treatment induced a significant decline in the values of serum testosterone, FSH, LH, GSH, GSH-Px and SOD. It also increased the values of serum MDA and LDH and spermatic arrest. While, the administration of Se-NPs orally before MSG treatment resulted in a decline in the values of serum MDA and LDH, an elevation in the values of serum GSH, GSH-PX and SOD, testosterone, FSH, LH and reappearance of sperm. <b>Conclusion:</b> The use of Se-NPs as a protector exhibited more improvement in values of estimated hormones and oxidative stress markers than using it as a therapy.
<;b>;背景和目的:</b>;增味剂味精(MSG)主要用于亚洲和西非菜肴,尤其是西非和亚洲菜肴。然而,由于其在许多食品中基本上没有标签,因此可能会无意中过度使用这种食品添加剂。本研究的目的是了解硒纳米颗粒如何影响MSG对雄性白化大鼠睾丸的毒性<;b>;材料和方法:</b>;将35只Wistar雄性大鼠分为5组:G1:对照大鼠,G2:接受0.4mg/kg<;sup>;1</sup>;b.wt.,口服,G3:以4g kg的日剂量注射MSG<;sup>;1</sup>;b.wt.,腹膜内(IP),G4:连续7天每天口服剂量的Se-NPs,并在第7天接受第一剂量的MSG IP 4gkg<;sup>;1</sup>;b.wt.,然后接受两种治疗直到研究结束,G5:每天口服剂量4gkg<;sup>;1</sup>;MSG,随后以0.4mg/kg<;sup>;1</sup>;实验持续了28天。测定血清睾酮、卵泡刺激素(FSH)、促黄体生成素(LH)、血清脂质过氧化(MDA)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和乳酸脱氢酶(LDH)水平,分离睾丸样品进行组织学分析<;b>;结果:</b>;MSG处理使血清睾酮、FSH、LH、GSH、GSH-Px和SOD值显著下降。提高血清MDA和LDH的含量,使精子停止活动。而在MSG处理前口服Se-NPs可导致血清MDA和LDH值下降,血清GSH、GSH-PX和SOD值升高,睾酮、FSH、LH值升高,精子出现<;b>;结论:</b>;使用Se-NPs作为保护剂比使用它作为治疗剂在估计的激素和氧化应激标志物的值方面表现出更多的改善。
期刊介绍:
Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.