Development of Monoclonal Antibodies Specific to Either CD300A^R111 or CD300A^Q111 or Both.

Q3 Medicine Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-10-01 DOI:10.1089/mab.2023.0017

Hitoshi Koizumi, Chigusa Nakahashi-Oda, Wenxin Lyu, Yumi Yamashita-Kanemaru, Keiji Tabuchi, Kazuko Shibuya, Akira Shibuya

{"title":"Development of Monoclonal Antibodies Specific to Either CD300AR111 or CD300AQ111 or Both.","authors":"Hitoshi Koizumi, Chigusa Nakahashi-Oda, Wenxin Lyu, Yumi Yamashita-Kanemaru, Keiji Tabuchi, Kazuko Shibuya, Akira Shibuya","doi":"10.1089/mab.2023.0017","DOIUrl":null,"url":null,"abstract":"CD300A is a member of the CD300 immunoglobulin (Ig)-like receptor family consisting of eight molecules in humans, all of which contain one Ig-like domain in the extracellular portion. Upon binding its ligand phosphatidylserine or phosphatidylethanolamine, CD300A mediates an inhibitory signal through the immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic portion. The CD300 family molecules are highly homologous to each other. In addition, CD300A has a single nucleotide polymorphism (rs2272111), which is a nonsense mutation encoding glutamine (CD300AQ111) instead of arginine (CD300AR111) at residue 111 in the Ig-like domain of CD300A. In this study, we successfully generated monoclonal antibodies (mAbs) specific to either CD300AR111 or CD300AQ111 or both. These mAbs are useful for the analysis of CD300A genotype by flow cytometry and the development of an antibody drug for the treatment of various diseases.","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/mab.2023.0017","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

CD300A is a member of the CD300 immunoglobulin (Ig)-like receptor family consisting of eight molecules in humans, all of which contain one Ig-like domain in the extracellular portion. Upon binding its ligand phosphatidylserine or phosphatidylethanolamine, CD300A mediates an inhibitory signal through the immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic portion. The CD300 family molecules are highly homologous to each other. In addition, CD300A has a single nucleotide polymorphism (rs2272111), which is a nonsense mutation encoding glutamine (CD300A^Q111) instead of arginine (CD300A^R111) at residue 111 in the Ig-like domain of CD300A. In this study, we successfully generated monoclonal antibodies (mAbs) specific to either CD300A^R111 or CD300A^Q111 or both. These mAbs are useful for the analysis of CD300A genotype by flow cytometry and the development of an antibody drug for the treatment of various diseases.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

特异于CD300AR111或CD300AQ111或两者的单克隆抗体的开发。

CD300A是CD300免疫球蛋白（Ig）样受体家族的成员，该家族在人类中由八个分子组成，所有这些分子在细胞外部分都包含一个Ig样结构域。在结合其配体磷脂酰丝氨酸或磷脂酰乙醇胺时，CD300A通过细胞质部分中基于免疫受体酪氨酸的抑制基序介导抑制信号。CD300家族分子彼此高度同源。此外，CD300A具有单核苷酸多态性（rs2272111），这是在CD300A的Ig样结构域的残基111编码谷氨酰胺（CD300AQ111）而不是精氨酸（CD300AR111）的无义突变。在这项研究中，我们成功地产生了对CD300AR111或CD300AQ111或两者都有特异性的单克隆抗体（mAb）。这些mAb可用于通过流式细胞术分析CD300A基因型和开发用于治疗各种疾病的抗体药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Medicine-Immunology and Allergy

CiteScore

4.80

自引率

0.00%

发文量