A network pharmacology approach to explore pharmacological mechanisms of Asparagus racemosus for ameliorative effect in epilepsy and comorbid progressive memory dysfunction.

In silico pharmacology Pub Date : 2023-10-27 eCollection Date: 2023-01-01 DOI:10.1007/s40203-023-00169-x

Baldeep Kaur, Sandeep Kumar, Arvinder Kaur, Rajesh Kumar Goel

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Abstract

Background: Network pharmacology approach has been observed a powerful tool to predict underlying complex pharmacological mechanism of herbs. Asparagus racemosus has been reported to show ameliorative effects in treating epilepsy and comorbid memory dysfunction but mechanism of this amelioration is elusive. Hence a network pharmacology approach was employed to investigate the plausible mechanism of A. recemosus.

Methodology: : Bioactive compounds of A. racemosus were extracted based on the TCMSP, PCIDB, and BATMAN-TCM database. The potential targets of bioactive compounds were collected using target fishing. Epilepsy and comorbid dementia genes were collected from DISGENET. A PPI network among these targets was constructed using the intersecting key targets between herb targets and disease targets. Besides, DAVID bioinformatics resource was utilized for the pathway enrichment analysis on GO and KEGG. Ultimately, phytochemical compound-target genes-Pathways network has been assembled utilizing Cytoscape to decipher the mechanism of the herb.

Results: The network analysis revealed that 5 targets (CASP3, TNF, VEGFA, PTGS2 and CNR1) might be the key therapeutic targets of asparagus on Epilepsy comorbid Alzheimer's disease. Based on high connectivity, four hub compounds with the highest connectivity were noted and it includes Shatavarin V, Sarsasapogenin, Shatavarin IX, and Shatavarin VI. A total of 19 KEGG terms were enriched as the potential pathways of A. racemosus in Epilepsy comorbid Alzheimer's disease.

Conclusion: This study envisaged the pharmacological and molecular mechanism of A. racemosus against epilepsy comorbid Alzheimer's disease and put forward a strategy to uncover the mechanisms of Traditional Indian Medicine based on network pharmacology.

Supplementary information: The online version contains supplementary material available at 10.1007/s40203-023-00169-x.

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一种网络药理学方法，探索外消旋芦笋改善癫痫和共病进行性记忆功能障碍的药理学机制。

背景：网络药理学方法已被观察到是预测草药潜在复杂药理机制的有力工具。据报道，外消旋芦笋在治疗癫痫和共病记忆功能障碍方面表现出改善作用，但这种改善机制尚不清楚。因此，采用网络药理学的方法来研究a.recemosus的可能机制。方法：基于TCMSP、PCIDB和BATMAN-TCM数据库提取外消旋A.racemosus的生物活性化合物。利用靶标捕鱼法收集了生物活性化合物的潜在靶标。癫痫和共病性痴呆的基因来自DISGENET。利用草药靶标和疾病靶标之间的交叉关键靶标构建了这些靶标之间的PPI网络。此外，利用DAVID生物信息学资源对GO和KEGG进行了通路富集分析。最终，利用Cytoscape构建了植物化学化合物靶基因通路网络，以破译草药的机制。结果：网络分析显示，5个靶点（CASP3、TNF、VEGFA、PTGS2和CNR1）可能是芦笋治疗癫痫合并阿尔茨海默病的关键靶点。基于高连接性，发现了四种具有最高连接性的枢纽化合物，包括沙塔瓦林V、Sarsasapogen、沙塔瓦林IX和沙塔瓦兰VI。共有19个KEGG术语被富集为外消旋A.racemosus在癫痫合并阿尔茨海默病中的潜在途径。结论：本研究设想了外消旋A.racemosus对抗癫痫合并阿尔茨海默病的药理学和分子机制，并提出了一种基于网络药理学揭示印度传统医学机制的策略。补充信息：在线版本包含补充材料，可访问10.1007/s40203-023-00169-x。

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In silico pharmacology

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