[Evaluation of the lower urinary tract function using voiding spot assay in mice].

Q3 Medicine 生理学报 Pub Date : 2023-10-25
Chuang Luo, Jia-Li Yang, Juan Liu, Xiang Qiu, Xiang Xie, Huan Chen
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引用次数: 0

Abstract

The objective of present study was to develop a simple and reliable voiding spot assay (VSA) system to evaluate the lower urinary tract function of mice, and to establish it as a standardized protocol. Ultraviolet (UV) light was used to screen out the filter paper without autofluorescence and with optimal urine diffusion properties. Next, the appropriate wavelength of UV was determined based on the quality of the photographic image of urine spots on the filter paper. To confirm that the urine stain area on the filter paper was correlated with the amount of urine, a volume-area standard curve was constructed. The utility of this VSA system was validated using female wild-type C57BL/6J mice aged 12-13 weeks, and the data generated under identical procedural settings were compared among laboratories. Furthermore, this VSA system was employed to analyze the changes in voiding patterns in mice with urinary tract infections or transportation stress. No. 4 filter paper with a thickness of 0.7 mm was identified as the most suitable material for VSA, exhibiting no autofluorescence and facilitating optimal urine diffusion. The filter paper retained its integrity during the assay, and there was a linear correlation between urine volume and stained area under 365 nm UV light. Utilizing this VSA system, we determined that female wild-type C57BL/6J mice produced approximately 695.8 μL total urine and 5.5 primary voiding spots (PVS) with an average size of 126.4 μL/spot within 4-h period. Over 84% of PVS volumes ranged from 20 to 200 μL. Notably, PVS volumes of mice were similar across different laboratories. Mice with urinary tract infections or transportation stress exhibited significant changes in VSA parameters, including increased voiding frequency, PVS number, and decreased PVS volume. Therefore, this VSA system can be used to evaluate the urinary function of normal mice, as well as those with urinary tract infection or transportation stress.

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[使用小鼠排泄点测定法评估下尿路功能]。
本研究的目的是开发一种简单可靠的排泄点测定(VSA)系统来评估小鼠的下尿路功能,并将其建立为一种标准化方案。使用紫外线(UV)来筛选出没有自发荧光并且具有最佳尿液扩散特性的滤纸。接下来,基于滤纸上尿斑的摄影图像的质量来确定UV的适当波长。为了确认滤纸上的尿液染色面积与尿液量相关,构建了体积-面积标准曲线。使用12-13周龄的雌性野生型C57BL/6J小鼠验证了该VSA系统的实用性,并在实验室之间比较了在相同程序设置下产生的数据。此外,该VSA系统被用于分析患有尿路感染或运输应激的小鼠排尿模式的变化。厚度为0.7mm的4号滤纸被确定为最适合VSA的材料,其不表现出自发荧光并促进最佳尿液扩散。滤纸在测定过程中保持了其完整性,并且在365nm紫外线下尿量和染色面积之间存在线性相关性。利用该VSA系统,我们确定雌性野生型C57BL/6J小鼠在4小时内产生约695.8μL的总尿液和5.5个主要排泄点(PVS),平均大小为126.4μL/点。超过84%的PVS体积在20至200μL之间。值得注意的是,不同实验室的小鼠PVS体积相似。患有尿路感染或运输应激的小鼠表现出VSA参数的显著变化,包括排尿频率增加、PVS数量增加和PVS体积减少。因此,该VSA系统可用于评估正常小鼠以及患有尿路感染或运输应激的小鼠的尿功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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