Evaluation of Cytotoxicity and Antimicrobial Activity of Experimental Composites Containing Chitosan-Silver Oxide Particles Against Two Main Pathogenic Bacteria in Periodontal Disease.

IF 1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Protein and Peptide Letters Pub Date : 2024-01-01 DOI:10.2174/0109298665240242231016103321
Nahid Nasrabadi, Navid Ramezanian, Parisa Ghorbanian, Ali Forouzanfar, Hamideh Sadat Mohammadipour
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Abstract

Introduction: Bacterial biofilm is known as the main cause of periodontal disease. Generally, the anaerobic Gram-negative, such as Porphyromonas gingivalis and Fusobacterium nucleatum, are considered the most identified bacteria.

Objective: This study aimed to investigate the antimicrobial effect and cytotoxicity of two experimental composites containing chitosan-silver oxide (CH-Ag2O) particles.

Materials and methods: Four experimental groups, including Ag2O and CH, along with two composites of CH-Ag2O 20 and CH-Ag2O 60 mg, were prepared. Antimicrobial activity was performed against Porphyromonas gingivalis (ATCC#33277) and Fusobacterium nucleatum (ATCC#25586) using the agar dilution method. Moreover, the cytotoxicity assay was performed on human gingival fibroblasts (HGF) by the use of the MTT method. The obtained data were analyzed with descriptive methods, one-way ANOVA, and Tukey's LSD tests.

Results: The antibacterial activity of both composites was higher than both CH and Ag2O, and the greatest antibacterial properties were presented in CH-Ag2O 60. In all three measurements (24, 48, and 72 h), the greatest cytotoxicity was seen in Ag2O, followed by CH, CH-Ag2O 20, and CHAg2O 60 in descending order, respectively. The cytotoxicity of these components was related to the concentration and not to the time of exposure. The results showed that Ag2O in 3.7 and 7.5 μg/ml concentrations and CH-containing groups in 250 and 500 μg/ml were toxic to the cultured HGF.

Conclusion: The experimental composite containing CH-Ag2O 60 showed the greatest antibacterial properties against two periodontal pathogens evaluated. In order to clarify the clinical significance of composite cytotoxicity, further clinical studies are necessary.

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含壳聚糖-氧化银颗粒的实验复合物对牙周病中两种主要病原菌的细胞毒性和抗菌活性评价。
简介:细菌生物膜是引起牙周病的主要原因。一般来说,厌氧革兰氏阴性菌,如牙龈卟啉单胞菌和有核梭杆菌,被认为是鉴定最多的细菌。目的:研究两种含有壳聚糖-氧化银(CH-Ag2O)颗粒的实验复合材料的抗菌效果和细胞毒性。材料和方法:制备了四个实验组,包括Ag2O和CH,以及两种CH-Ag2O 20和CH-Ag2O60mg的复合材料。使用琼脂稀释法对牙龈卟啉单胞菌(ATCC#33277)和有核梭杆菌(ATCC#25586)进行抗菌活性。此外,采用MTT法对人牙龈成纤维细胞(HGF)进行细胞毒性测定。使用描述性方法、单因素方差分析和Tukey的LSD检验对获得的数据进行分析。结果:两种复合材料的抗菌活性均高于CH和Ag2O,其中CH-Ag2O60的抗菌性能最好。在所有三次测量(24、48和72小时)中,Ag2O的细胞毒性最大,依次为CH、CH-Ag2O 20和CH-Ag2O60。这些成分的细胞毒性与浓度有关,而与暴露时间无关。结果表明,3.7和7.5μg/ml浓度的Ag2O和250和500μg/ml的含CH组对培养的HGF具有毒性。为了阐明复合细胞毒性的临床意义,有必要进行进一步的临床研究。
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来源期刊
Protein and Peptide Letters
Protein and Peptide Letters 生物-生化与分子生物学
CiteScore
2.90
自引率
0.00%
发文量
98
审稿时长
2 months
期刊介绍: Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis
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