Oxylipin profiling by LC-ESI-MS/MS in canine serum and plasma to investigate ovulation-specific changes

IF 2.5 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Prostaglandins & other lipid mediators Pub Date : 2023-10-31 DOI:10.1016/j.prostaglandins.2023.106790

Margareta Ramström , Bodil S. Holst , Richard Lindberg , Malin L. Nording

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Abstract

New biomarkers that are directly associated with canine ovulation would be of value to ensure mating on optimal days of heat. In this study, canine plasma and serum were analyzed with liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to quantify a broad range of oxylipins for the purpose of developing a method for biomarker discovery studies in canine reproduction. A majority of the 67 oxylipins probed for were detected at comparable levels in both sample types, but more oxylipins at higher concentrations were detected in serum than in plasma. Nine of the oxylipins were detected in a pilot study of serum at levels that significantly differed (p ≤ 0.1) between time-points before (n = 10), during (n = 10) and after (n = 10) ovulation, and might serve as putative biomarkers for canine ovulation. One oxylipin (20-HETE) was significantly altered after adjusting for multiple comparisons. In conclusion, the results showed that the LC-ESI-MS/MS method was suitable for quantification of canine oxylipins, revealing important similarities and differences between plasma and serum profiles as well as preliminary ovulation-specific changes in a subset of the investigated oxylipins.

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通过LC-ESI-MS/MS分析犬血清和血浆中的氧脂蛋白，以研究排卵特异性变化。

与狗排卵直接相关的新生物标志物将有助于确保在最佳高温条件下交配。在这项研究中，用液相色谱-电喷雾电离串联质谱法（LC-ESI-MS/MS）分析了犬的血浆和血清，以量化广泛的oxylipins，目的是开发一种用于犬繁殖中生物标志物发现研究的方法。在两种样本类型中，检测到的67种氧基化酶中的大多数都在相当的水平上检测到，但在血清中检测到的浓度更高的氧基化蛋白比在血浆中检测到更多。在一项血清初步研究中检测到9种oxylipins，其水平存在显著差异（p

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来源期刊

Prostaglandins & other lipid mediators 生物-生化与分子生物学

CiteScore

5.80

自引率

3.40%

发文量

审稿时长

2 months

期刊介绍： Prostaglandins & Other Lipid Mediators is the original and foremost journal dealing with prostaglandins and related lipid mediator substances. It includes basic and clinical studies related to the pharmacology, physiology, pathology and biochemistry of lipid mediators. Prostaglandins & Other Lipid Mediators invites reports of original research, mini-reviews, reviews, and methods articles in the basic and clinical aspects of all areas of lipid mediator research: cell biology, developmental biology, genetics, molecular biology, chemistry, biochemistry, physiology, pharmacology, endocrinology, biology, the medical sciences, and epidemiology. Prostaglandins & Other Lipid Mediators also accepts proposals for special issue topics. The Editors will make every effort to advise authors of the decision on the submitted manuscript within 3-4 weeks of receipt.