Drug resistance and the genotypic characteristics of rpoB and katG in rifampicin- and/or isoniazid-resistant Mycobacterium tuberculosis isolates in central Vietnam.
Thi Binh Nguyen Nguyen, Thi Kieu Diem Nguyen, Van Hue Trương, Thi Tuyet Ngoc Tran, van Bao Thang Phan, Thi Tuyen Nguyen, Hoang Bach Nguyen, Viet Quynh Tram Ngo, Van Tuan Mai, Paola Molicotti
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引用次数: 0
Abstract
Background: Tuberculosis (TB) and drug-resistant TB (DR-TB) are national health burdens in Vietnam. In this study, we investigated the prevalence of rifampicin (RIF) and/or isoniazid (isonicotinic acid hydrazide, INH) resistance in patients with suspected TB, and applied appropriate techniques to help rapidly target DR-TB.
Methods: In total, 1,547 clinical specimens were collected and cultured using the BACTEC MGIT system (Becton Dickinson and Co.). A resazurin microtiter assay (REMA) was used to determine the proportions of RIF and/or INH resistance. A real-time polymerase chain reaction panel with TaqMan probes was employed to identify the mutations of rpoB and katG associated with DR-TB in clinical isolates. Genotyping of the identified mutations was also performed.
Results: A total of 468 Mycobacterium tuberculosis isolates were identified using the REMA. Of these isolates, 106 (22.6%) were found to be resistant to 1 or both antibiotics. Of the resistant isolates, 74 isolates (69.8%) were resistant to isoniazid (INH) only, while 1 isolate (0.94%) was resistant to RIF only. Notably, 31 isolates (29.24%) were resistant to both antibiotics. Of the 41 phenotypically INH-resistant isolates, 19 (46.3%) had the Ser315Thr mutation. There were 8 different rpoB mutations in 22 (68.8%) of the RIF-resistant isolates. The most frequently detected mutations were at codons 531 (37.5%), 526 (18.8%), and 516 (6.3%).
Conclusion: To help prevent new cases of DR-TB in Vietnam, it is crucial to gain a comprehensive understanding of the genotypic DR-TB isolates.
背景：结核病（TB）和耐药结核病（DR-TB）是越南的国民健康负担。在本研究中，我们调查了疑似结核病患者中利福平（RIF）和/或异烟肼（异烟肼，INH）耐药性的患病率，并应用适当的技术帮助快速靶向DR-TB。方法：总的来说，使用BACTEC MGIT系统（Becton Dickinson and Co.）收集并培养1547个临床标本。使用雷沙唑林微量滴定法（REMA）测定RIF和/或INH耐药性的比例。使用TaqMan探针的实时聚合酶链反应小组来鉴定临床分离株中与DR-TB相关的rpoB和katG的突变。还对已鉴定的突变进行了基因分型。结果：利用REMA共鉴定出468株结核分枝杆菌。在这些分离株中，发现106株（22.6%）对一种或两种抗生素都有耐药性。在耐药菌株中，74株（69.8%）仅对异烟肼（INH）耐药，1株（0.94%）仅对RIF耐药。值得注意的是，31个分离株（29.24%）对这两种抗生素都有耐药性。在41个表型INH抗性菌株中，19个（46.3%）具有Ser315Thr突变。在22个（68.8%）RIF耐药菌株中，有8个不同的rpoB突变。最常见的突变发生在密码子531（37.5%）、526（18.8%）和516（6.3%）。结论：为了防止越南出现新的DR-TB病例，全面了解基因型DR-TB分离株至关重要。
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