7,8-Dihydroxy Flavone Induces Apoptosis via Upregulation of Caspase-3 in Human Hepatocarcinoma Cell.

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL Eurasian Journal of Medicine Pub Date : 2023-10-01 DOI:10.5152/eurasianjmed.2023.22283
Gülsüm Abuşoğlu, Mukaddes İrem Durmuş, Serdar Karakurt
{"title":"7,8-Dihydroxy Flavone Induces Apoptosis via Upregulation of Caspase-3 in Human Hepatocarcinoma Cell.","authors":"Gülsüm Abuşoğlu, Mukaddes İrem Durmuş, Serdar Karakurt","doi":"10.5152/eurasianjmed.2023.22283","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>7,8-Dihydroxyflavone, a tyrosine kinase receptor agonist, is a flavonoid that has recently gained the attention of researchers due to its anticancer properties. Nevertheless, molecular pathways of 7,8-dihydroxyflavone for hepatocarcinoma are uncertain. Our aim was to identify the impact of 7,8-dihydroxyflavone on human hepatocarcinoma.</p><p><strong>Material and methods: </strong>Human hepatocarcinoma cell line-7 cells were used as human hepatocarcinoma cells, and 7,8-dihydroxyflavone was applied to the cells at various doses. The cytotoxic and apoptotic effects of 7,8-dihydroxyflavone were determined with Alamar Blue and flow cytometry. The properties of 7,8-dihydroxyflavone on the mRNA expressions related with Bcl-2, Bax, cleaved-caspase-3 genes, and protein expressions were determined via quantitative real-time polymerase chain reaction and western blot analysis, respectively.</p><p><strong>Results: </strong>7,8-Dihydroxyflavone-enhanced cell death in human hepatocarcinoma cell line-7 via the overexpression of cleaved-caspase-3 (P=.003) and decreased Bcl-2 (P=.038) protein levels. Furthermore, cleavedcaspase-3 mRNA overexpression (P=.001) markedly led to 7,8-dihydroxyflavone-induced apoptosis.</p><p><strong>Conclusion: </strong>7,8-Dihydroxyflavone could promote apoptotic cell death by modulating caspase pathways and suppressing antiapoptotic protein. These characteristics may mediate to clinical practice of 7,8-dihydroxyflavone for prevention and therapy of hepatocarcinoma.</p>","PeriodicalId":53592,"journal":{"name":"Eurasian Journal of Medicine","volume":"55 3","pages":"199-203"},"PeriodicalIF":0.9000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10724746/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eurasian Journal of Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5152/eurasianjmed.2023.22283","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: 7,8-Dihydroxyflavone, a tyrosine kinase receptor agonist, is a flavonoid that has recently gained the attention of researchers due to its anticancer properties. Nevertheless, molecular pathways of 7,8-dihydroxyflavone for hepatocarcinoma are uncertain. Our aim was to identify the impact of 7,8-dihydroxyflavone on human hepatocarcinoma.

Material and methods: Human hepatocarcinoma cell line-7 cells were used as human hepatocarcinoma cells, and 7,8-dihydroxyflavone was applied to the cells at various doses. The cytotoxic and apoptotic effects of 7,8-dihydroxyflavone were determined with Alamar Blue and flow cytometry. The properties of 7,8-dihydroxyflavone on the mRNA expressions related with Bcl-2, Bax, cleaved-caspase-3 genes, and protein expressions were determined via quantitative real-time polymerase chain reaction and western blot analysis, respectively.

Results: 7,8-Dihydroxyflavone-enhanced cell death in human hepatocarcinoma cell line-7 via the overexpression of cleaved-caspase-3 (P=.003) and decreased Bcl-2 (P=.038) protein levels. Furthermore, cleavedcaspase-3 mRNA overexpression (P=.001) markedly led to 7,8-dihydroxyflavone-induced apoptosis.

Conclusion: 7,8-Dihydroxyflavone could promote apoptotic cell death by modulating caspase pathways and suppressing antiapoptotic protein. These characteristics may mediate to clinical practice of 7,8-dihydroxyflavone for prevention and therapy of hepatocarcinoma.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
7,8-二羟基黄酮通过上调人肝癌细胞中的半胱氨酸蛋白酶-3诱导细胞凋亡。
目的:7,8-二羟基黄酮是一种酪氨酸激酶受体激动剂,近年来因其抗癌特性而受到研究者的关注。然而,7,8-二羟基黄酮治疗肝癌的分子途径尚不确定。我们的目的是确定7,8-二羟基黄酮对人肝癌的影响。材料与方法:以人肝癌细胞系7细胞为材料,采用不同剂量的7,8-二羟基黄酮对细胞进行处理。用Alamar蓝和流式细胞仪测定了7,8-二羟基黄酮的细胞毒性和凋亡作用。通过实时定量聚合酶链反应和蛋白质印迹分析,分别测定了7,8-二羟基黄酮对Bcl-2、Bax、裂解的胱天蛋白酶-3基因相关mRNA表达和蛋白质表达的影响。结果:7,8-二羟基黄酮通过过表达裂解的胱天蛋白酶-3(P=0.003)和降低Bcl-2(P=0.038)蛋白水平,增强了人肝癌细胞系-7的细胞死亡。此外,裂解的半胱氨酸蛋白酶-3 mRNA过表达(P=0.001)显著导致7,8-二羟基黄酮诱导的细胞凋亡。结论:7,8-二羟基黄酮可通过调节caspase通路和抑制抗凋亡蛋白来促进细胞凋亡。这些特征可能介导7,8-二羟基黄酮预防和治疗肝癌的临床实践。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Eurasian Journal of Medicine
Eurasian Journal of Medicine Medicine-Medicine (all)
CiteScore
1.90
自引率
6.70%
发文量
59
审稿时长
16 weeks
期刊介绍: Eurasian Journal of Medicine (Eurasian J Med) is an international, scientific, open access periodical published by independent, unbiased, and triple-blinded peer-review principles. The journal is the official publication of Atatürk University School of Medicine and published triannually in February, June, and October. The publication language of the journal is English. The aim of the Eurasian Journal of Medicine is to publish original research papers of the highest scientific and clinical value in all medical fields. The Eurasian J Med also includes reviews, editorial short notes and letters to the editor that either as a comment related to recently published articles in our journal or as a case report. The target audience of the journal includes researchers, physicians and healthcare professionals who are interested or working in in all medical disciplines.
期刊最新文献
Clinical and Sociodemographic Characteristics that May Affect Delays in Child Sexual Abuse Disclosures: Ten Years in Practice. Healthy Lifestyle Behaviors of Research Assistants and Final-Year Students in the Medical Faculty. Current Developments in the Treatment of Postpartum Depression: Zuranolone. Intravenous Lipid Emulsion Therapy in Drug Overdose and Poisoning: An Updated Review. Novel Alternative Block for Scoliosis Surgery: Serratus Posterior Superior Plane Block, Does Not Need to Visualize the Transverse Process.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1