Erythropoietin inhibits neutrophil extracellular traps formation to ameliorate lung injury in a pneumonia model.

IF 2.5 4区医学 Q3 ALLERGY Allergologia et immunopathologia Pub Date : 2023-11-01 eCollection Date: 2023-01-01 DOI:10.15586/aei.v51i6.980

Sheng Ye, Wei Li, Jinghui Yang, Xiang Xue, Jiao Chen, Wei Zhao, Lei Jiang, Ling Jia

{"title":"Erythropoietin inhibits neutrophil extracellular traps formation to ameliorate lung injury in a pneumonia model.","authors":"Sheng Ye, Wei Li, Jinghui Yang, Xiang Xue, Jiao Chen, Wei Zhao, Lei Jiang, Ling Jia","doi":"10.15586/aei.v51i6.980","DOIUrl":null,"url":null,"abstract":"Background: Severe pneumonia is a kind of disease that develops from lung inflammation, and new drugs are still required to treat the same. Erythropoietin (EPO) is widely used to treat anemia in patients. However, there are fewer studies on the role of EPO in neutrophil extracellular trappings (NETs) and pneumonia, and the mechanism is unclear.Objective: To investigate the possible effects of EPO on the formation of NETs and progression of pneumonia.Methods: Mice pneumonia model was induced by tracheal lipopolysaccharide (LPS) administration. Hematoxylin and eosin (H&E) staining and automatic blood cell analysis were performed in this model to confirm the effects of EPO on lung injury. Flow cytometry, enzyme-linked immunosorbent serological assay, and immunostaining assay were conducted to detect the effects of EPO on the inflammation as well as formation of NETs in mice. Immunoblot was further conducted to confirm the mechanism.Results: EPO alleviated LPS-induced lung injury. EPO reduced the release of inflammatory factors induced by LPS. In addition, EPO inhibited the formation of NETs. Mechanically, EPO inhibited tumor necrosis factor (TNF) receptor associated factor 2 (TRAF2)/nuclear factor kappa-B (NF-κB) activity in mouse models, and therefore suppressed the progression of pneumonia.Conclusion: EPO inhibited formation of NETs to ameliorate lung injury in a pneumonia model, and could serve as a drug of pneumonia.","PeriodicalId":7536,"journal":{"name":"Allergologia et immunopathologia","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Allergologia et immunopathologia","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.15586/aei.v51i6.980","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"ALLERGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Severe pneumonia is a kind of disease that develops from lung inflammation, and new drugs are still required to treat the same. Erythropoietin (EPO) is widely used to treat anemia in patients. However, there are fewer studies on the role of EPO in neutrophil extracellular trappings (NETs) and pneumonia, and the mechanism is unclear.

Objective: To investigate the possible effects of EPO on the formation of NETs and progression of pneumonia.

Methods: Mice pneumonia model was induced by tracheal lipopolysaccharide (LPS) administration. Hematoxylin and eosin (H&E) staining and automatic blood cell analysis were performed in this model to confirm the effects of EPO on lung injury. Flow cytometry, enzyme-linked immunosorbent serological assay, and immunostaining assay were conducted to detect the effects of EPO on the inflammation as well as formation of NETs in mice. Immunoblot was further conducted to confirm the mechanism.

Results: EPO alleviated LPS-induced lung injury. EPO reduced the release of inflammatory factors induced by LPS. In addition, EPO inhibited the formation of NETs. Mechanically, EPO inhibited tumor necrosis factor (TNF) receptor associated factor 2 (TRAF2)/nuclear factor kappa-B (NF-κB) activity in mouse models, and therefore suppressed the progression of pneumonia.

Conclusion: EPO inhibited formation of NETs to ameliorate lung injury in a pneumonia model, and could serve as a drug of pneumonia.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

红细胞生成素抑制中性粒细胞外陷阱的形成以改善肺炎模型中的肺损伤。

背景：严重肺炎是一种由肺部炎症发展而来的疾病，目前仍需要新药来治疗。促红细胞生成素（EPO）被广泛用于治疗贫血患者。然而，关于EPO在中性粒细胞外捕获（NETs）和肺炎中的作用的研究较少，其机制尚不清楚。目的：探讨EPO对NETs形成和肺炎进展的可能影响。方法：采用气管脂多糖（LPS）诱导小鼠肺炎模型。在该模型中进行苏木精和伊红（H&E）染色和自动血细胞分析，以证实EPO对肺损伤的影响。通过流式细胞术、酶联免疫吸附血清学试验和免疫染色试验检测EPO对小鼠炎症和NETs形成的影响。进一步进行免疫印迹以证实该机制。结果：EPO减轻了LPS诱导的肺损伤。EPO减少LPS诱导的炎症因子的释放。此外，EPO抑制NETs的形成。EPO在机制上抑制了小鼠模型中肿瘤坏死因子（TNF）受体相关因子2（TRAF2）/核因子κB（NF-κB）的活性，从而抑制了肺炎的进展。结论：EPO可抑制NETs的形成，改善肺炎模型肺损伤，可作为治疗肺炎的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.