Effects of irisin and exercise on adropin and betatrophin in a new metabolic syndrome model.

IF 1.6 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-11-01 Epub Date: 2024-01-09 DOI:10.1080/10520295.2023.2276205

Suna Aydin, Faruk Kilinc, Kader Ugur, Mustafa Ata Aydin, Mehmet Hanifi Yalcin, Tuncay Kuloglu, Nalan Kaya Tektemur, Serdal Albayrak, Elif Emre, Meltem Yardim, Ramazan Fazil Akkoc, Serhat Hancer, İbrahim Sahin, Vedat Cinar, Taner Akbulut, Selcuk Demircan, Bahri Evren, Berrin Tarakci Gencer, Aziz Aksoy, Merve Yilmaz Bozoglan, İsa Aydemir, Suleyman Aydin

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Abstract

Metabolic syndrome (MetS) is a prevalent public health problem. Uric acid (UA) is increased by MetS. We investigated whether administration of UA and 10% fructose (F) would accelerate MetS formation and we also determined the effects of irisin and exercise. We used seven groups of rats. Group 1 (control); group 2 (sham); group 3 (10% F); group 4 (1% UA); group 5 (2% UA); group 6 (10% F + 1% UA); and Group 7, (10% F + 2% UA). After induction of MetS (groups 3 -7), Group 3 was divided into three subgroups: 3A, no further treatment; 3B, irisin treatment; 3C, irisin treatment + exercise. Group 4, 1% UA, which was divided into three subgroups: 4A, no further treatment; 4B, irisin treatment; 4C, Irisin treatment + exercise. Group 5, 2% UA, which was divided into three subgroups: 5A, no further treatment; 5B, irisin treatment; 5C, irisin treatment + exercise. Group 6, 10% F + 1% UA, which was divided into three subgroups: 6A, no further treatment; 6B, irisin treatment; 6C, irisin treatment + exercise. Group 7, 10% F + 2% UA, which was divided into three subgroups: 7A, no further treatment; 7B, irisin treatment; 7C, irisin treatment + exercise., İrisin was administered 10 ng/kg irisin intraperitoneally on Monday, Wednesday, Friday, Sunday each week for 1 month. The exercise animals (in addition to irisin treatment) also were run on a treadmill for 45 min on Monday, Wednesday, Friday, Sunday each week for 1 month. The rats were sacrificed and samples of liver, heart, kidney, pancreas, skeletal muscles and blood were obtained. The amounts of adropin (ADR) and betatrophin in the tissue supernatant and blood were measured using an ELISA method. Immunohistochemistry was used to detect ADR and betatrophin expression in situ in tissue samples. The duration of these experiments varied from 3 and 10 weeks. The order of development of MetS was: group 7, 3 weeks; group 6, 4 weeks; group 5, 6 weeks; group 4, 7 weeks; group 3, 10 weeks. Kidney, liver, heart, pancreas and skeletal muscle tissues are sources of adropin and betatrophin. In these tissues and in the circulation, adropin was decreased significantly, while betatrophin was increased significantly due to MetS; irisin + exercise reversed this situation. We found that the best method for creating a MetS model was F + UA2 supplementation. Our method is rapid and simple. Irisin + exercise was best for preventing MetS.

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在一种新的代谢综合征模型中，鸢尾素和运动对adropin和betatropin的影响。

代谢综合征（MetS）是一个普遍存在的公共卫生问题。MetS可增加尿酸（UA）。我们研究了UA和10%果糖（F）的给药是否会加速MetS的形成，并确定了鸢尾素和运动的影响。我们用了七组老鼠。第1组（对照组）；第2组（假手术组）；第3组（10%F）；第4组（1%UA）；第5组（2%UA）；第6组（10%F+1%UA）；第7组（10%F+2%UA）。MetS诱导后（第3-7组），第3组分为三个亚组：3A，不进一步治疗；3B、鸢尾素治疗；3C，鸢尾素治疗+运动。第4组，1%UA，分为三个亚组：4A，不进一步治疗；4B、鸢尾素治疗；4C，Irisin治疗+运动。第5组，2%UA，分为三个亚组：5A，不进一步治疗；5B，鸢尾素治疗；5C，鸢尾素治疗+运动。第6组，10%F+1%UA，分为三个亚组：6A，不进一步治疗；6B，鸢尾素治疗；6C，鸢尾素治疗+运动。第7组，10%F+2%UA，分为三个亚组：7A，不进一步治疗；7B，鸢尾素治疗；7C，鸢尾素治疗+运动。，伊里辛于每周周一、周三、周五、周日腹膜内注射10纳克/公斤鸢尾素，持续1个月。运动动物（除了鸢尾素治疗外）也在跑步机上每周周一、周三、周五、周日运行45分钟，持续1个月。处死大鼠，获取肝脏、心脏、肾脏、胰腺、骨骼肌和血液样本。使用ELISA方法测量组织上清液和血液中的adropin（ADR）和betatropin的量。免疫组织化学法检测组织样品中ADR和β-营养素的原位表达。这些实验的持续时间从3周到10周不等。MetS的发展顺序为：第7组，3周；第6组，4周；第5、6周组；第4组，7周；第3组，10周。肾脏、肝脏、心脏、胰腺和骨骼肌组织是阿曲平和β营养素的来源。在这些组织和循环中，adropin显著减少，而betatropin由于MetS而显著增加；irisin+运动扭转了这种局面。我们发现创建MetS模型的最佳方法是补充F+UA2。我们的方法快速而简单。Irisin+运动是预防MetS的最佳方法。

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来源期刊

Biotechnic & Histochemistry 生物-生物工程与应用微生物

CiteScore

3.40

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.