Reconstitution of actin-based cellular processes: Why encapsulation changes the rules

IF 4.5 3区 生物学 Q2 CELL BIOLOGY European journal of cell biology Pub Date : 2023-10-30 DOI:10.1016/j.ejcb.2023.151368
Fabina Binth Kandiyoth, Alphée Michelot
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引用次数: 0

Abstract

While in vitro reconstitution of cellular processes is progressing rapidly, the encapsulation of biomimetic systems to reproduce the cellular environment is a major challenge. Here we review the difficulties, using reconstitution of processes dependent on actin polymerization as an example. Some of the problems are purely technical, due to the need for engineering strategies to encapsulate concentrated solutions in micrometer-sized compartments. However, other significant issues arise from the reduction of experimental volumes, which alters the chemical evolution of these non-equilibrium systems. Important parameters to consider for successful reconstitutions are the amount of each component, their consumption and renewal rates to guarantee their continuous availability.

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肌动蛋白细胞过程的重组:为什么包封改变了规则。
虽然细胞过程的体外重建进展迅速,但封装仿生系统以再现细胞环境是一个重大挑战。在这里,我们回顾了困难,以肌动蛋白聚合过程的重建为例。一些问题纯粹是技术性的,因为需要工程策略将浓缩的解决方案封装在微米大小的隔间中。然而,实验体积的减少也带来了其他重大问题,这改变了这些非平衡系统的化学演化。成功重建需要考虑的重要参数是每种成分的数量、消耗量和更新率,以确保其持续可用。
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来源期刊
European journal of cell biology
European journal of cell biology 生物-细胞生物学
CiteScore
7.30
自引率
1.50%
发文量
80
审稿时长
38 days
期刊介绍: The European Journal of Cell Biology, a journal of experimental cell investigation, publishes reviews, original articles and short communications on the structure, function and macromolecular organization of cells and cell components. Contributions focusing on cellular dynamics, motility and differentiation, particularly if related to cellular biochemistry, molecular biology, immunology, neurobiology, and developmental biology are encouraged. Manuscripts describing significant technical advances are also welcome. In addition, papers dealing with biomedical issues of general interest to cell biologists will be published. Contributions addressing cell biological problems in prokaryotes and plants are also welcome.
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