CD41+ extracellular vesicles produced by avian thrombocytes contain microRNAs

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-11-05 DOI:10.1111/gtc.13078
Kenkichi Sugimoto, Takamasa Nishikawa, Toshie Sugiyama
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Abstract

Avians have thrombocytes in their blood circulation rather than mammalian platelets. However, many details of thrombocyte characteristics have not been determined. Here, chicken thrombocytes were isolated, and extracellular vesicle (EV) production was investigated. The thrombocyte-specific markers cd41 and cd61 were expressed in the yolk sac at 24 h. According to the embryonic developmental stage, the cd41-expressing tissues changed from the yolk sac to the bone marrow and spleen. Accordingly, the bone marrow and spleen were the main tissues producing thrombocytes in adult chickens. Avian thrombocytes were separated from adult spleen cells through a combination of discontinuous density gradient centrifugation, phagocytic cell removal, and fluorescence-activated cell sorting. Isolated thrombocytes produced CD41+ EVs (CD41+ EVs), and the CD41+ EVs also expressed CD9. Microarray analysis revealed that CD41+ EVs contain many microRNAs. Macrophage lines (RAW264.7) phagocytosed CD41+ EVs, and their phagocytosis and migration activity were suppressed. Microarray analysis also revealed that EVs altered gene expression in macrophages. These data indicated that the CD41+ EV was a carrier of microRNAs produced from thrombocytes and affected the cell characteristics of the received cells. Therefore, the CD41+ EVs of avians worked as a communication tool.

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禽类血小板产生的CD41+细胞外小泡含有微小RNA。
鸟类的血液循环中有血小板,而不是哺乳动物的血小板。然而,血小板特征的许多细节尚未确定。在此,分离了鸡的血小板,并研究了细胞外小泡(EV)的产生。24时卵黄囊中表达了血小板特异性标志物cd41和cd61 h.根据胚胎发育阶段,表达cd41的组织从卵黄囊向骨髓和脾脏转变。因此,骨髓和脾脏是成年鸡产生血小板的主要组织。通过不连续密度梯度离心、吞噬细胞去除和荧光激活细胞分选相结合的方法,从成年脾细胞中分离出鸡血小板。分离的血小板产生CD41+EV(CD41+EV),并且CD41+EV也表达CD9。微阵列分析显示CD41+EVs含有许多微小RNA。巨噬细胞系(RAW264.7)吞噬CD41+EVs,其吞噬和迁移活性受到抑制。微阵列分析还显示EVs改变了巨噬细胞中的基因表达。这些数据表明CD41+EV是由血小板产生的微小RNA的载体,并影响所接收细胞的细胞特性。因此,鸟类的CD41+EV起到了沟通工具的作用。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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