Didymella acutilobae sp. nov. Causing Leaf Spot and Stem Rot in Angelica acutiloba.

IF 2 4区生物学 Q2 AGRONOMY Mycobiology Pub Date : 2023-10-12 eCollection Date: 2023-01-01 DOI:10.1080/12298093.2023.2254052

Gyo-Bin Lee, Ki Deok Kim, Weon-Dae Cho, Wan-Gyu Kim

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Abstract

During disease surveys of Angelica acutiloba plants in Korea, leaf spot symptoms were observed in a field in Andong in July 2019, and stem rot symptoms in vinyl greenhouses in Yangpyeong in April 2020. Incidence of leaf spot and stem rot of the plants ranged from 10 to 20% and 5 to 30%, respectively. Morphological and cultural characteristics of fungal isolates from the leaf spot and stem rot symptoms fitted into those of the genus Phoma. Molecular phylogenetic analyses of two single-spore isolates from the symptoms using concatenated sequences of LSU, ITS, TUB2, and RPB2 genes authenticated an independent cluster from other Didymella (anamorph: Phoma) species. Moreover, the isolates showed different morphological and cultural characteristics in comparison to closely related Didymella species. These discoveries confirmed the novelty of the isolates. Pathogenicity of the novel Didymella species isolates was substantiated on leaves and stems of A. acutiloba through artificial inoculation. Thus, this study reveals that Didymella acutilobae sp. nov. causes leaf spot and stem rot in Angelica acutiloba.

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尖叶Didymela acutilobae sp.nov.引起尖叶当归叶斑和茎腐病的研究。

在韩国对尖叶当归植物进行疾病调查期间，2019年7月在安东的一块田地里观察到了叶斑症状，2020年4月在杨平的乙烯基温室里观察到茎腐症状。叶斑病和茎腐病的发生率分别为10-20%和5-30%。从叶斑病和茎腐病症状中分离的真菌的形态和培养特征符合Phoma属的特征。使用LSU、ITS、TUB2和RPB2基因的串联序列对来自症状的两个单孢子分离株进行分子系统发育分析，验证了来自其他Didymela（变形：Phoma）物种的独立聚类。此外，与亲缘关系密切的Didymela物种相比，分离株表现出不同的形态和文化特征。这些发现证实了分离物的新颖性。通过人工接种，证实了新的Didymela分离株在尖叶甲叶和茎上的致病性。因此，本研究揭示了尖叶Didymela acutilobae sp.nov.引起尖叶当归叶斑和茎腐病的原因。

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来源期刊

Mycobiology AGRONOMYMYCOLOGY-MYCOLOGY

CiteScore

3.90

自引率

5.30%

发文量

审稿时长

22 weeks

期刊介绍： Mycobiology is an international journal devoted to the publication of fundamental and applied investigations on all aspects of mycology and their traditional allies. It is published quarterly and is the official publication of the Korean Society of Mycology. Mycobiology publishes reports of basic research on fungi and fungus-like organisms, including yeasts, filamentous fungi, lichen fungi, oomycetes, moulds, and mushroom. Topics also include molecular and cellular biology, biochemistry, metabolism, developmental biology, environmental mycology, evolution, ecology, taxonomy and systematics, genetics/genomics, fungal pathogen and disease control, physiology, and industrial biotechnology using fungi.