Photodynamic inactivation by hypericin-P123 on azole-resistant isolates of the Trichophyton rubrum complex as planktonic cells and biofilm.

Photodiagnosis and photodynamic therapy Pub Date : 2023-12-01 Epub Date: 2023-11-01 DOI:10.1016/j.pdpdt.2023.103875
Juliana Aparecida Fernandes, Pollyanna Cristina Vicenzi Conrado, Brenda Serenini Perina, Ana Carolina Vieira de Oliveira, Glaucia Sayuri Arita, Isis Regina Grenier Capoci, Renato Sonchini Gonçalves, Wilker Caetano, Terezinha Inez Estivalet Svidzinski, Erika Seki Kioshima Cotica, Patrícia de Souza Bonfim-Mendonça
{"title":"Photodynamic inactivation by hypericin-P123 on azole-resistant isolates of the Trichophyton rubrum complex as planktonic cells and biofilm.","authors":"Juliana Aparecida Fernandes, Pollyanna Cristina Vicenzi Conrado, Brenda Serenini Perina, Ana Carolina Vieira de Oliveira, Glaucia Sayuri Arita, Isis Regina Grenier Capoci, Renato Sonchini Gonçalves, Wilker Caetano, Terezinha Inez Estivalet Svidzinski, Erika Seki Kioshima Cotica, Patrícia de Souza Bonfim-Mendonça","doi":"10.1016/j.pdpdt.2023.103875","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The Trichophyton rubrum complex comprises the majority of dermatophyte fungi (DM) responsible for chronic cases of onychomycosis, which is treated with oral or topical antifungals. However, owing to antifungal resistance, alternative therapies, such as photodynamic therapy (PDT), are needed. This study investigated the frequency of the T. rubrum species complex in onychomycosis cases in the northwestern region of Paraná state, Brazil, and evaluated the efficacy of (PDT) using P123-encapsulated hypericin (Hyp-P123) on clinical isolates of T. rubrum in the planktonic cell and biofilm forms.</p><p><strong>Material and methods: </strong>The frequency of the T. rubrum complex in onychomycosis cases from 2017 to 2021 was evaluated through a data survey of records from the Laboratory of Medical Mycology (LEPAC) of the State University of Maringa (UEM). To determine the effect of PDT-Hyp-P123 on planktonic cells of T. rubrum isolates, 1 × 10<sup>5</sup> conidia/mL were treated with ten different concentrations of Hyp-P123 and then irradiated with 37.8 J/cm<sup>2</sup>. Antibiofilm activity of PDT-Hyp-P123 was tested against T. rubrum biofilm in the adhesion phase (3 h), evaluated 72 h after irradiation (37.8 J/cm<sup>2</sup>), and the mature biofilm (72 h), evaluated immediately after irradiation. In this context, three different parameters were evaluated: cell viability, metabolic activity and total biomass.</p><p><strong>Results: </strong>The T. rubrum species complex was the most frequently isolated DM in onychomycosis cases (approximately 80 %). A significant reduction in fungal growth was observed for 75 % of the clinical isolates tested with a concentration from 0.19 μmol/L Hyp-P123, and 56.25 % had complete inhibition of fungal growth (fungicidal action); while all isolates were azole-resistant. The biofilm of T. rubrum isolates (TR0022 and TR0870) was inactivated in both the adhesion phase and the mature biofilm.</p><p><strong>Conclusion: </strong>PDT-Hyp-P123 had antifungal and antibiofilm activity on T. rubrum, which is an important dermatophyte responsible for onychomycosis cases.</p>","PeriodicalId":94170,"journal":{"name":"Photodiagnosis and photodynamic therapy","volume":" ","pages":"103875"},"PeriodicalIF":0.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photodiagnosis and photodynamic therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.pdpdt.2023.103875","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/1 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: The Trichophyton rubrum complex comprises the majority of dermatophyte fungi (DM) responsible for chronic cases of onychomycosis, which is treated with oral or topical antifungals. However, owing to antifungal resistance, alternative therapies, such as photodynamic therapy (PDT), are needed. This study investigated the frequency of the T. rubrum species complex in onychomycosis cases in the northwestern region of Paraná state, Brazil, and evaluated the efficacy of (PDT) using P123-encapsulated hypericin (Hyp-P123) on clinical isolates of T. rubrum in the planktonic cell and biofilm forms.

Material and methods: The frequency of the T. rubrum complex in onychomycosis cases from 2017 to 2021 was evaluated through a data survey of records from the Laboratory of Medical Mycology (LEPAC) of the State University of Maringa (UEM). To determine the effect of PDT-Hyp-P123 on planktonic cells of T. rubrum isolates, 1 × 105 conidia/mL were treated with ten different concentrations of Hyp-P123 and then irradiated with 37.8 J/cm2. Antibiofilm activity of PDT-Hyp-P123 was tested against T. rubrum biofilm in the adhesion phase (3 h), evaluated 72 h after irradiation (37.8 J/cm2), and the mature biofilm (72 h), evaluated immediately after irradiation. In this context, three different parameters were evaluated: cell viability, metabolic activity and total biomass.

Results: The T. rubrum species complex was the most frequently isolated DM in onychomycosis cases (approximately 80 %). A significant reduction in fungal growth was observed for 75 % of the clinical isolates tested with a concentration from 0.19 μmol/L Hyp-P123, and 56.25 % had complete inhibition of fungal growth (fungicidal action); while all isolates were azole-resistant. The biofilm of T. rubrum isolates (TR0022 and TR0870) was inactivated in both the adhesion phase and the mature biofilm.

Conclusion: PDT-Hyp-P123 had antifungal and antibiofilm activity on T. rubrum, which is an important dermatophyte responsible for onychomycosis cases.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
hypericin-P123对红色毛癣菌复合物的唑抗性分离株作为浮游细胞和生物膜的光动力学失活。
简介:红色毛癣菌复合体包括大多数导致甲真菌病慢性病例的皮肤癣菌(DM),可通过口服或局部抗真菌药物治疗。然而,由于抗真菌耐药性,需要替代疗法,如光动力疗法(PDT)。本研究调查了巴西巴拉那州西北部地区甲真菌病病例中红色念珠菌物种复合物的频率,并评估了使用P123包埋金丝桃素(Hyp-P123)的PDT对浮游细胞和生物膜形式的红色念珠菌临床分离株的疗效。材料和方法:通过对马里加州立大学医学真菌学实验室(LEPAC)记录的数据调查,评估了2017年至2021年甲真菌病病例中红曲霉复合体的频率。为了测定PDT-Hyp-P123对红色念珠菌分离株浮游细胞的影响,用10种不同浓度的Hyp-P1123处理1×105分生孢子/mL,然后用37.8J/cm2照射。在粘附阶段(3小时)测试PDT-Hyp-P123的抗生物膜活性,在照射后72小时评估(37.8J/cm2),并在照射后立即评估成熟生物膜(72小时)。在此背景下,评估了三个不同的参数:细胞活力、代谢活性和总生物量。结果:红曲霉属复合体是甲真菌病病例中最常见的DM(约80%)。在浓度为0.19μmol/L的Hyp-P123的试验中,75%的临床分离株的真菌生长显著减少,56.25%的分离株对真菌生长具有完全抑制作用(杀真菌作用);而所有分离株均对唑具有抗性。红曲霉分离株(TR0022和TR0870)的生物膜在粘附期和成熟生物膜中均失活。结论:PDT-Hyp-P123对红霉菌具有抗真菌和抗菌膜活性,红霉菌是引起甲真菌病的重要皮肤真菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Highly malignant tumor cells accumulate less PpIX and enhanced cell dormancy increases PpIX accumulation. PHOTODIAGNOSIS WITH DEEP LEARNING: A GAN AND AUTOENCODER-BASED APPROACH FOR DIABETIC RETINOPATHY DETECTION. Comparative analysis of photodynamic therapy and conization for cervical high-grade squamous intraepithelial lesion: a systematic review and meta-analysis. A novel vaginal manipulator for identifying vaginal canal separation line by visible and near-infrared transillumination light: PHARUS Pipe. In vitro effect of photodynamic therapy with two photosensitizers on Streptococcus mutans load around metal and ceramic brackets bonded to enamel.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1