Considerations for colorblind individuals on selecting colorimetric or fluorescent dye assay outcomes.

IF 2.2 4区 工程技术 Q3 BIOCHEMICAL RESEARCH METHODS BioTechniques Pub Date : 2023-12-01 Epub Date: 2023-11-09 DOI:10.2144/btn-2023-0041
Kirstyn Loyva, Erik Hofmeister, Fiona Georgousi, Constance Roderick, Rebecca Cole
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Abstract

A disadvantage of colorimetric detection in nucleic acid amplification assays is the possibility that a colorblind individual may interpret colors differently than observers with full-color vision. Using an isothermal amplification assay, the ability of colorblind individuals to distinguish between positive and negative results for four dyes was tested. Five individuals with self-reported colorblindness and four with full-color vision reported their observations of the color of the solution. Although colorblind individuals may accurately interpret assay results, they were often not accurate in reporting the color. Hydroxynaphthol blue was the most problematic dye, and both phenol red and SYBR™ green were less troublesome. Consideration for colorblind individuals is warranted when developing an assay and training staff in its performance.

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色盲个体选择比色或荧光染料测定结果的注意事项。
核酸扩增测定中比色检测的缺点是色盲个体可能与具有全色视觉的观察者不同地解释颜色。使用等温扩增试验,测试了色盲个体区分四种染料的阳性和阴性结果的能力。五名自我报告的色盲患者和四名全色视力患者报告了他们对溶液颜色的观察结果。尽管色盲个体可以准确地解释测定结果,但他们在报告颜色时往往不准确。羟基萘蓝是最有问题的染料,酚红和SYBR™ 绿色就不那么麻烦了。在开发检测方法和培训工作人员时,有必要考虑色盲个体。
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来源期刊
BioTechniques
BioTechniques 工程技术-生化研究方法
CiteScore
4.40
自引率
0.00%
发文量
68
审稿时长
3.3 months
期刊介绍: BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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