Evaluation of human papillomavirus DNA in colorectal cancer and adjacent mucosal tissue samples.

IF 3.1 2区医学 Q3 IMMUNOLOGY Infectious Agents and Cancer Pub Date : 2023-11-08 DOI:10.1186/s13027-023-00552-5

Luisa Galati, Purnima Gupta, Antonio Tufaro, Mariarosaria Marinaro, Concetta Saponaro, David Israel Escobar Marcillo, Donato Loisi, Rajdip Sen, Alexis Robitaille, Rosario N Brancaccio, Cyrille Cuenin, Sandrine McKay-Chopin, Angelo Virgilio Paradiso, Václav Liška, Pavel Souček, Francesco Alfredo Zito, David J Hughes, Massimo Tommasino, Tarik Gheit

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Abstract

Background: Although the role of viral agents, such as human papillomavirus (e.g. HPV16, HPV18) in colorectal cancer (CRC) has been previously investigated, results remain inconclusive.

Methods: To further evaluate the involvement of oncogenic HPV types in CRC, 40 frozen neoplastic and 40 adjacent colonic tissues collected from Italian patients were analyzed by Luminex-based assays that detect a broad spectrum of HPV types, i.e. Alpha (n = 21), Beta (n = 46) and Gamma HPVs (n = 52). In addition, 125 frozen CRC samples and 70 surrounding mucosal tissues were collected from Czech patients and analyzed by broad spectrum PCR protocols: (i) FAP59/64, (ii) FAPM1 and (iii) CUT combined with Next Generation Sequencing (NGS).

Results: Using Luminex-basedassays, DNA from HPV16 was detected in 5% (2/40) CRC tissues from Italian patients. One HPV16 DNA-positive CRC case was subsequently confirmed positive for E6*I mRNA. Cutaneous beta HPV types were detected in 10% (4/40) adjacent tissues only, namely HPV111 (n = 3) and HPV120 (n = 1), while gamma HPV168 (n = 1) and HPV199 (n = 1) types were detected in adjacent and in tumor tissues, respectively. The NGS analysis of the CRC Czech samples identified HPV sequences from mucosal alpha-3 (HPV89), alpha-7 (HPV18, 39, 68 and 70) and alpha-10 species (HPV11), as well as cutaneous beta-1 (HPV20, 24, 93, 98, 105,124) beta-2 (HPV23), beta-3 (HPV49) and gamma-1 species (HPV205).

Conclusions: Our findings indicate that HPV types belonging to the mucosal alpha, and the 'cutaneous' beta and gamma genera can be detected in the colonic mucosal samples with a low prevalence rate and a low number of HPV reads by Luminex and NGS, respectively. However, additional studies are required to corroborate these findings.

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人乳头瘤病毒DNA在结直肠癌癌症和邻近粘膜组织样本中的评估。

背景：尽管之前已经研究过病毒制剂，如人乳头瘤病毒（如HPV16、HPV18）在癌症（CRC）中的作用，但结果仍不确定。方法：为了进一步评估致癌HPV类型在CRC中的作用，通过基于Luminex的检测方法分析了从意大利患者收集的40个冷冻肿瘤组织和40个邻近结肠组织，该检测方法检测了广泛的HPV类型，即α（n = 21），贝塔（n = 46）和伽玛HPV（n = 52）。此外，从捷克患者身上采集了125份冷冻CRC样本和70份周围粘膜组织，并通过广谱PCR方案进行分析：（i）FAP59/64，（ii）FAPM1和（iii）CUT结合下一代测序（NGS）。一例HPV16 DNA阳性CRC病例随后被证实E6*I mRNA阳性。仅在10%（4/40）的邻近组织中检测到皮肤β型HPV，即HPV111（n = 3）和HPV120（n = 1），而γHPV168（n = 1）和HPV199（n = 1）分别在邻近组织和肿瘤组织中检测到类型。CRC捷克样本的NGS分析确定了来自粘膜α-3（HPV89）、α-7（HPV18、39、68和70）和α-10物种（HPV11）以及皮肤β-1（HPV20、24、93、98、105124）β-2（HPV23）、β-3（HPV49）和γ-1物种（HPV205）的HPV序列。结论：我们的发现表明，Luminex和NGS分别在结肠粘膜样本中检测到“皮肤”β属和γ属，其患病率和HPV读数较低。然而，还需要更多的研究来证实这些发现。

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来源期刊

Infectious Agents and Cancer ONCOLOGY-IMMUNOLOGY

CiteScore

5.80

自引率

2.70%

发文量

期刊介绍： Infectious Agents and Cancer is an open access, peer-reviewed online journal that encompasses all aspects of basic, clinical, epidemiological and translational research providing an insight into the association between chronic infections and cancer. The journal welcomes submissions in the pathogen-related cancer areas and other related topics, in particular: • HPV and anogenital cancers, as well as head and neck cancers; • EBV and Burkitt lymphoma; • HCV/HBV and hepatocellular carcinoma as well as lymphoproliferative diseases; • HHV8 and Kaposi sarcoma; • HTLV and leukemia; • Cancers in Low- and Middle-income countries. The link between infection and cancer has become well established over the past 50 years, and infection-associated cancer contribute up to 16% of cancers in developed countries and 33% in less developed countries. Preventive vaccines have been developed for only two cancer-causing viruses, highlighting both the opportunity to prevent infection-associated cancers by vaccination and the gaps that remain before vaccines can be developed for other cancer-causing agents. These gaps are due to incomplete understanding of the basic biology, natural history, epidemiology of many of the pathogens that cause cancer, the mechanisms they exploit to cause cancer, and how to interrupt progression to cancer in human populations. Early diagnosis or identification of lesions at high risk of progression represent the current most critical research area of the field supported by recent advances in genomics and proteomics technologies.