Isolation, Identification and Screening of Saharan Actinomycete Strain Streptomyces fimbriatus AC31 Endowed with Antimicrobial Activity.

IF 1.5 Q3 MEDICINE, RESEARCH & EXPERIMENTAL International Journal of Molecular and Cellular Medicine Pub Date : 2023-01-01 DOI:10.22088/IJMCM.BUMS.12.1.51
Omar Khirennas, Slimane Mokrani, Belkacem Behira, Noureddine Bouras, El Hadj Driche, Ouahiba Moumen
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Abstract

The increasing global public health concern of antimicrobial resistance (AMR) necessitates exploration of natural antimicrobial agents as potential alternatives. This study aimed to investigate antimicrobial activities of Saharan actinomycetes, with specific focus on the strain Streptomyces fimbriatus AC31, that holds promising potential as an alternative to combat AMR. In this context, 32 actinomycetes were isolated from El Atteuf (Ghardaïa), Algeria. Isolates obtained were characterized morphologically and biochemically. Screened isolate was identified by 16S rRNA gene sequencing. Classification of actinomycete isolates was carried out by UPGMA (Unweighted Pair Group Method with Arithmetic Mean). Then, they were screened for their antimicrobial activity by cross-streak method. Identification of 32 isolates revealed 5 genera: Streptomyces (65.63%), Nocardia (9.38%), Streptosporangium (9.38%), Nocardiopsis (9.38%) and Actinomadura (6.25%). According to the biochemical and physiological characteristics, UPGMA classified the isolates in 4 phenons. A number of 24 (75.00%) isolates were active against Gram-positive bacteria, 21 (65.63%) isolates were effective against Gram-negative bacteria, and 25 (78.13%) isolates inhibited Candida albicans. Screened strain Streptomyces fimbriatus AC31 showed highest antagonistic activity and revealed an inhibition zones of 41, 38, 41, 42, and 44 mm, against B. subtilis (ATCC 6633), E. coli (ATCC 8739), S. typhimurium (ATCC 13331), S. aureus (ATCC 6538) and C. albicans (ATCC 10231), respectively. Phylogenetic identification of the AC 31 isolate using 16S rRNA gene sequence showed similarity of 100% with Streptomyces fimbriatus NBRC 15411T. Actinomycete isolates characterized in this study were endowed with antimicrobial activity against various pathogenic microorganisms that could be used efficiently in developing new antimicrobial substances.

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撒哈拉放线菌菌毛链霉菌AC31菌株的分离、鉴定和筛选。
抗微生物耐药性(AMR)日益引起全球公共卫生关注,因此有必要探索天然抗微生物剂作为潜在的替代品。本研究旨在研究撒哈拉放线菌的抗菌活性,特别关注具有对抗AMR潜力的菌毛链霉菌AC31菌株。在这种情况下,从阿尔及利亚的El Atteuf(Ghardaïa)分离到32种放线菌。对获得的分离物进行了形态学和生物化学表征。通过16S rRNA基因测序鉴定筛选出的分离物。用算术平均未加权对群法对放线菌进行分类。然后,用十字条纹法对其抗菌活性进行筛选。32个分离株共鉴定出5个属:链霉菌属(65.63%)、诺卡氏菌属(9.38%)、链孢囊菌属(93.8%)、诺卡氏菌属和放线菌属(6.25%)。24个(75.00%)分离株对革兰氏阳性菌有活性,21个(65.63%)分离株对白念珠菌有抑制作用,25个(78.13%)分离株抑制白色念珠菌。筛选菌株伞状链霉菌AC31显示出最高的拮抗活性,并显示出分别对枯草芽孢杆菌(ATCC6633)、大肠杆菌(ATCC8739)、鼠伤寒杆菌(ATCC113331)、金黄色葡萄球菌(ATCC6538)和白色念珠菌(ATCC10231)的41、38、41、42和44mm的抑制区。使用16S rRNA基因序列对AC 31分离株的系统发育鉴定显示,其与伞状链霉菌NBRC 15411T的相似性为100%。本研究中鉴定的放线菌分离株具有对各种病原微生物的抗菌活性,可有效用于开发新的抗菌物质。
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期刊介绍: The International Journal of Molecular and Cellular Medicine (IJMCM) is a peer-reviewed, quarterly publication of Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. The journal covers all cellular & molecular biology and medicine disciplines such as the genetic basis of disease, biomarker discovery in diagnosis and treatment, genomics and proteomics, bioinformatics, computer applications in human biology, stem cells and tissue engineering, medical biotechnology, nanomedicine, cellular processes related to growth, death and survival, clinical biochemistry, molecular & cellular immunology, molecular and cellular aspects of infectious disease and cancer research. IJMCM is a free access journal. All open access articles published in IJMCM are distributed under the terms of the Creative Commons Attribution CC BY. The journal doesn''t have any submission and article processing charges (APCs).
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