Anne Meinzinger, Áron Zsigmond, Péter Horváth, Alexandra Kellenberger, Katalin Paréj, Tiziano Tallone, Beáta Flachner, Marcell Cserhalmi, Zsolt Lőrincz, Sándor Cseh, Doron Shmerling
{"title":"RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System.","authors":"Anne Meinzinger, Áron Zsigmond, Péter Horváth, Alexandra Kellenberger, Katalin Paréj, Tiziano Tallone, Beáta Flachner, Marcell Cserhalmi, Zsolt Lőrincz, Sándor Cseh, Doron Shmerling","doi":"10.1155/2023/7121512","DOIUrl":null,"url":null,"abstract":"<p><p>Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this study, a novel inducible system, the <i>RuX system</i>, was created using a mutant ligand-binding domain of the glucocorticoid receptor (CS1/CD), used together with various genetic elements such as the Gal4 DNA-binding domain or Cre recombinase. The RuX system is shown to be capable of over 1000-fold inducibility, has flexible applications, and is offered for use in cell cultures.</p>","PeriodicalId":39084,"journal":{"name":"International Journal of Cell Biology","volume":"2023 ","pages":"7121512"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630016/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2023/7121512","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this study, a novel inducible system, the RuX system, was created using a mutant ligand-binding domain of the glucocorticoid receptor (CS1/CD), used together with various genetic elements such as the Gal4 DNA-binding domain or Cre recombinase. The RuX system is shown to be capable of over 1000-fold inducibility, has flexible applications, and is offered for use in cell cultures.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
