The potential protective role of taurine against 5-fluorouracil-induced nephrotoxicity in adult male rats

Q2 Medicine Experimental and Toxicologic Pathology Pub Date : 2017-06-14 DOI:10.1016/j.etp.2017.01.012

Hany N. Yousef, Hanaa R. Aboelwafa

{"title":"The potential protective role of taurine against 5-fluorouracil-induced nephrotoxicity in adult male rats","authors":"Hany N. Yousef, Hanaa R. Aboelwafa","doi":"10.1016/j.etp.2017.01.012","DOIUrl":null,"url":null,"abstract":"<div>Nephrotoxicity is common with the use of the chemotherapeutic agent 5-Fluorouracil (5-FU). The current study aimed to investigate the probable protective effect of taurine (TAU) against 5-FU-induced nephrotoxicity in rats using biochemical, histological and ultrastructural approaches. Twenty-four rats were equally divided into control, TAU, 5-FU and 5-FU  <!-->TAU groups.5-FU significantly elevated levels of blood urea nitrogen (BUN), creatinine, and uric acid; while it reduced activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). Also, 5-FU induced significant elevation in malondialdehyde (MDA) levels accompanied with marked decline in γ-glutamyltranspeptidase (GGT) and alkaline phosphatase (AP) levels in kidney tissues. These biochemical alterations were accompanied by histopathological changes marked by destruction of the normal renal structure, in addition to ultrastructural alterations represented by thickened and irregular glomerular basement membranes, congested glomerular capillaries, damaged lining fenestrated endothelium, mesangial cells hyperplasia with expanded mesangial matrix, and distorted podocyte’s processes. Also, the proximal (PCT) and distal (DCT) convoluted tubules showed thickened basement membranes, destructed apical microvilli and loss of basal infoldings of their epithelial cells.Administration of TAU to 5-FU-treated rats reversed most of the biochemical, histological, and ultrastructural alterations. These results indicate that TAU has a protective effect against 5-FU-induced nephrotoxicity.</div>","PeriodicalId":50465,"journal":{"name":"Experimental and Toxicologic Pathology","volume":"69 5","pages":"Pages 265-274"},"PeriodicalIF":0.0000,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.etp.2017.01.012","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and Toxicologic Pathology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0940299316301737","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 21

Abstract

Nephrotoxicity is common with the use of the chemotherapeutic agent 5-Fluorouracil (5-FU). The current study aimed to investigate the probable protective effect of taurine (TAU) against 5-FU-induced nephrotoxicity in rats using biochemical, histological and ultrastructural approaches. Twenty-four rats were equally divided into control, TAU, 5-FU and 5-FU + TAU groups.

5-FU significantly elevated levels of blood urea nitrogen (BUN), creatinine, and uric acid; while it reduced activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). Also, 5-FU induced significant elevation in malondialdehyde (MDA) levels accompanied with marked decline in γ-glutamyltranspeptidase (GGT) and alkaline phosphatase (AP) levels in kidney tissues. These biochemical alterations were accompanied by histopathological changes marked by destruction of the normal renal structure, in addition to ultrastructural alterations represented by thickened and irregular glomerular basement membranes, congested glomerular capillaries, damaged lining fenestrated endothelium, mesangial cells hyperplasia with expanded mesangial matrix, and distorted podocyte’s processes. Also, the proximal (PCT) and distal (DCT) convoluted tubules showed thickened basement membranes, destructed apical microvilli and loss of basal infoldings of their epithelial cells.

Administration of TAU to 5-FU-treated rats reversed most of the biochemical, histological, and ultrastructural alterations. These results indicate that TAU has a protective effect against 5-FU-induced nephrotoxicity.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

牛磺酸对5-氟尿嘧啶引起的成年雄性大鼠肾毒性的潜在保护作用

肾毒性常见于化疗药物5-氟尿嘧啶（5-FU）的使用。本研究旨在通过生物化学、组织学和超微结构的方法研究牛磺酸（TAU）对5-FU诱导的大鼠肾毒性的可能保护作用。24只大鼠平均分为对照组、TAU组、5-FU组和5-FU+TAU组。5-FU显著升高血尿素氮（BUN）、肌酐和尿酸水平；同时降低超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性。此外，5-FU诱导肾组织中丙二醛（MDA）水平显著升高，同时γ-谷氨酰转肽酶（GGT）和碱性磷酸酶（AP）水平显著下降。这些生化变化伴随着以破坏正常肾脏结构为标志的组织病理学变化，此外还有超微结构变化，表现为肾小球基底膜增厚和不规则、肾小球毛细血管充血、衬里开窗内皮受损、系膜细胞增生伴系膜基质膨胀，和扭曲的足细胞过程。此外，近端（PCT）和远端（DCT）曲小管的基底膜增厚，顶端微绒毛破坏，上皮细胞的基底信息丢失。对5-FU处理的大鼠施用TAU逆转了大部分生化、组织学和超微结构的改变。这些结果表明TAU对5-FU诱导的肾毒性具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Experimental and Toxicologic Pathology 医学-病理学

CiteScore

2.08

自引率

0.00%

发文量

审稿时长

5.3 weeks

期刊介绍： Cessation. The international multidisciplinary journal is devoted to the publication of studies covering the whole range of experimental research on disease processes and toxicology including cell biological investigations. Its aim is to support progress in the interdisciplinary cooperation of researchers working in pathobiology, toxicology, and cell biology independent of the methods applied. During the past decades increasing attention has been paid to the importance of toxic influence in the pathogenesis of human and animal diseases. This is why Experimental and Toxicologic Pathology meets the urgent need for an interdisciplinary journal felt by a wide variety of experts in medicine and biology, including pathologists, toxicologists, biologists, physicians, veterinary surgeons, pharmacists, and pharmacologists working in academic, industrial or clinical institutions.