Regulation of Glycosyl-phosphoinositides in Mammalian Cells

Abstract

Glycosyl-phosphoinositide molecules have both structural and functional roles in mammalian cells. These glycophospholipids can serve as membrane anchors for cell surface proteins or as precursors for the generation of second messengers in hormone action. Methodology for analysis of the synthesis and metabolism of these molecules is outlined. Tissue culture cells are used for experiments involving labeling with radioactive precursors. After exposure to hormones, glycosyl-phosphoinositides and their metabolites can be analyzed by a combination of thin-layer and high-performance liquid chromatography.